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Microbiology 150 (2004), 2527-2534; DOI  10.1099/mic.0.27071-0
© 2004 Society for General Microbiology

Killer toxin of Pichia membranifaciens and its possible use as a biocontrol agent against grey mould disease of grapevine

A. Santos and D. Marquina

Department of Microbiology, Biology Faculty, Complutense University of Madrid, Madrid 28040, Spain

Correspondence
D. Marquina
dommarq{at}bio.ucm.es

The use of Pichia membranifaciens CYC 1106 killer toxin against Botrytis cinerea was investigated. This strain exerted a broad-specificity killing action against other yeasts and fungi. At pH 4, optimal killer activity was observed at temperatures up to 20 °C. At 25 °C the toxic effect was reduced to 70 %. The killer activity was higher in acidic medium. Above about pH 4·5 activity decreased sharply and was barely noticeable at pH 6. The killer toxin protein from P. membranifaciens CYC 1106 was purified to electrophoretic homogeneity. SDS-PAGE of the purified killer protein indicated an apparent molecular mass of 18 kDa. Killer toxin production was stimulated in the presence of non-ionic detergents. The toxin concentrations present in the supernatant during optimal production conditions exerted a fungicidal effect on a strain of B. cinerea. The symptoms of infection and grey mould observed in Vitis vinifera plants treated with B. cinerea were prevented in the presence of purified P. membranifaciens killer toxin. The results obtained suggest that P. membranifaciens CYC 1106 killer toxin is of potential use in the biocontrol of B. cinerea.

Abbreviations: Brij-58, polyoxyethylene 20 cetyl ether




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A. Santos, M. del Mar Alvarez, M. S. Mauro, C. Abrusci, and D. Marquina
The Transcriptional Response of Saccharomyces cerevisiae to Pichia membranifaciens Killer Toxin
J. Biol. Chem., December 23, 2005; 280(51): 41881 - 41892.
[Abstract] [Full Text] [PDF]


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