| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Biochemistry, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa
2 Laboratory of Fundamental and Applied Microbiology, LCEE UMR CNRS 6008, University of Poitiers, 40, avenue du Recteur Pineau, 86022 Poitiers Cedex, France
3 The Royal Veterinary and Agricultural University, Department of Dairy and Food Science, Centre for Advanced Food Studies, LMC, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark
Correspondence
Yann Héchard
yann.hechard{at}univ-poitiers.fr
Sensitivity to class IIa bacteriocins from lactic acid bacteria was recently associated with the mannose phosphotransferase system (PTS) permease, 
, in Listeria monocytogenes. To assess the involvement of this protein complex in class IIa bacteriocin activity, the mptACD operon, encoding 
, was heterologously expressed in an insensitive species, namely Lactococcus lactis, using the NICE double plasmid system. Upon induction of the cloned operon, the recombinant Lc. lactis became sensitive to leucocin A. Pediocin PA-1 and enterocin A also showed inhibitory activity against Lc. lactis cultures expressing mptACD. Furthermore, the role of the three genes of the mptACD operon was investigated. Derivative plasmids containing various combinations of these three genes were made from the parental mptACD plasmid by divergent PCR. The results showed that expression of mptC alone is sufficient to confer sensitivity to class IIa bacteriocins in Lc. lactis.
These authors contributed equally to this work.
This article has been cited by other articles:
|
|
 |
|
  P. M. Swe, G. M. Cook, J. R. Tagg, and R. W. Jack Mode of action of dysgalacticin: a large heat-labile bacteriocin J. Antimicrob. Chemother., April 1, 2009; 63(4): 679 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Xue and K. W. Miller Regulation of the mpt Operon in Listeria innocua by the ManR Protein Appl. Envir. Microbiol., September 1, 2007; 73(17): 5648 - 5652. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tominaga and Y. Hatakeyama Development of Innovative Pediocin PA-1 by DNA Shuffling among Class IIa Bacteriocins Appl. Envir. Microbiol., August 15, 2007; 73(16): 5292 - 5299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Calvez, A. Rince, Y. Auffray, H. Prevost, and D. Drider Identification of new genes associated with intermediate resistance of Enterococcus faecalis to divercin V41, a pediocin-like bacteriocin Microbiology, May 1, 2007; 153(5): 1609 - 1618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. F. Nes, D. B. Diep, and H. Holo Bacteriocin Diversity in Streptococcus and Enterococcus J. Bacteriol., February 15, 2007; 189(4): 1189 - 1198. [Full Text] [PDF]
|
|
|
|
 |
|
 D. B. Diep, M. Skaugen, Z. Salehian, H. Holo, and I. F. Nes Common mechanisms of target cell recognition and immunity for class II bacteriocins PNAS, February 13, 2007; 104(7): 2384 - 2389. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Deutscher, C. Francke, and P. W. Postma How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria Microbiol. Mol. Biol. Rev., December 1, 2006; 70(4): 939 - 1031. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Erni The mannose transporter complex: an open door for the macromolecular invasion of bacteria. J. Bacteriol., October 1, 2006; 188(20): 7036 - 7038. [Full Text] [PDF]
|
|
|
|
|
|
S. Bieler, F. Silva, C. Soto, and D. Belin Bactericidal Activity of both Secreted and Nonsecreted Microcin E492 Requires the Mannose Permease. J. Bacteriol., October 1, 2006; 188(20): 7049 - 7061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Drider, G. Fimland, Y. Hechard, L. M. McMullen, and H. Prevost The Continuing Story of Class IIa Bacteriocins Microbiol. Mol. Biol. Rev., June 1, 2006; 70(2): 564 - 582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Yebra, V. Monedero, M. Zuniga, J. Deutscher, and G. Perez-Martinez Molecular analysis of the glucose-specific phosphoenolpyruvate : sugar phosphotransferase system from Lactobacillus casei and its links with the control of sugar metabolism Microbiology, January 1, 2006; 152(1): 95 - 104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Johnsen, G. Fimland, and J. Nissen-Meyer The C-terminal Domain of Pediocin-like Antimicrobial Peptides (Class IIa Bacteriocins) Is Involved in Specific Recognition of the C-terminal Part of Cognate Immunity Proteins and in Determining the Antimicrobial Spectrum J. Biol. Chem., March 11, 2005; 280(10): 9243 - 9250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Xue, I. Hunter, T. Steinmetz, A. Peters, B. Ray, and K. W. Miller Novel Activator of Mannose-Specific Phosphotransferase System Permease Expression in Listeria innocua, Identified by Screening for Pediocin AcH Resistance Appl. Envir. Microbiol., March 1, 2005; 71(3): 1283 - 1290. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
