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1 Department of Biochemistry, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa
2 Laboratory of Fundamental and Applied Microbiology, LCEE UMR CNRS 6008, University of Poitiers, 40, avenue du Recteur Pineau, 86022 Poitiers Cedex, France
3 The Royal Veterinary and Agricultural University, Department of Dairy and Food Science, Centre for Advanced Food Studies, LMC, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark
Correspondence
Yann Héchard
yann.hechard{at}univ-poitiers.fr
Sensitivity to class IIa bacteriocins from lactic acid bacteria was recently associated with the mannose phosphotransferase system (PTS) permease, 
, in Listeria monocytogenes. To assess the involvement of this protein complex in class IIa bacteriocin activity, the mptACD operon, encoding 
, was heterologously expressed in an insensitive species, namely Lactococcus lactis, using the NICE double plasmid system. Upon induction of the cloned operon, the recombinant Lc. lactis became sensitive to leucocin A. Pediocin PA-1 and enterocin A also showed inhibitory activity against Lc. lactis cultures expressing mptACD. Furthermore, the role of the three genes of the mptACD operon was investigated. Derivative plasmids containing various combinations of these three genes were made from the parental mptACD plasmid by divergent PCR. The results showed that expression of mptC alone is sufficient to confer sensitivity to class IIa bacteriocins in Lc. lactis.
These authors contributed equally to this work.
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