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Microbiology 150 (2004), 2739-2749; DOI  10.1099/mic.0.27153-0
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Microbiology 150 (2004), 2739-2749; DOI  10.1099/mic.0.27153-0
© 2004 Society for General Microbiology

NblA is essential for phycobilisome degradation in Anabaena sp. strain PCC 7120 but not for development of functional heterocysts

Kerstin Baier1, Heike Lehmann1, Dirk Paul Stephan2 and Wolfgang Lockau1

1 Humboldt-Universität zu Berlin, Institut für Biologie, Biochemie der Pflanzen, Chausseestrasse 117, D-10115 Berlin, Germany
2 Universität Bielefeld, Fakultät für Biologie, Bio VIII Molekulare Zellphysiologie, PO Box 100131, D-33501 Bielefeld, Germany

Correspondence
Kerstin Baier
kerstin.baier{at}biologie.hu-berlin.de

Phycobilisomes (PBS) are the major light-harvesting complexes of cyanobacteria. These usually blue-coloured multiprotein assemblies are rapidly degraded when the organisms are starved for combined nitrogen. This proteolytic process causes a colour change of the cyanobacterial cells from blue-green to yellow-green (‘bleaching’). As is well documented for the unicellular, non-diazotrophic cyanobacteria Synechococcus elongatus PCC 7942 and Synechocystis sp. PCC 6803, a gene termed nblA plays a key role in PBS degradation. Filamentous, diazotrophic cyanobacteria like Anabaena adapt to nitrogen deprivation by differentiation of N2-fixing heterocysts. However, during the first hours after nitrogen deprivation all cells degrade their PBS. When heterocysts mature and nitrogenase becomes active, vegetative cells resynthesize their light-harvesting complexes while in heterocysts the phycobiliprotein content remains very low. Expression and function of nblA in Anabaena sp. PCC 7120 was investigated. This strain has two nblA homologous genes, one on the chromosome (nblA) and one on plasmid delta (nblA-p). Northern blot analysis indicated that only the chromosomal nblA gene is up-regulated upon nitrogen starvation. Mutants with interrupted nblA and nblA-p genes, respectively, grew on N2 and developed functional heterocysts. Mutant {Delta}nblA-p behaved like the wild-type. However, mutant {Delta}nblA was unable to degrade its PBS, which was most obvious in non-bleaching heterocysts. The results show that NblA, encoded by the chromosomal nblA gene, is required for PBS degradation in Anabaena but is not essential for heterocyst differentiation.

Abbreviations: Anabaena 7120, Anabaena sp. PCC 7120; Anabaena 29413, Anabaena variabilis ATCC 29413; PBS, phycobilisome(s); Synechococcus 7942, Synechococcus elongatus PCC 7942; Synechocystis 6803, Synechocystis sp. PCC 6803

The GenBank/EMBL accession number for the nblA sequence from Anabaena 29413 reported in this paper is AJ504665.




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