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Characterization of the Aspergillus parasiticus ¹²-desaturase gene: a role for lipid metabolism in the Aspergillus–seed interaction

¹ Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706, USA
² Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA
³ Southern Regional Research Center, Agricultural Research Service, US Department of Agriculture, New Orleans, LA 70124, USA

Correspondence
Nancy P. Keller
npk{at}plantpath.wisc.edu

In the mycotoxigenic oilseed pathogens Aspergillus flavus and Aspergillus parasiticus and the model filamentous fungus Aspergillus nidulans, unsaturated fatty acids and their derivatives act as important developmental signals that affect asexual conidiospore, sexual ascospore and/or sclerotial development. To dissect the relationship between lipid metabolism and fungal development, an A. parasiticus ¹²-desaturase mutant that was unable to convert oleic acid to linoleic acid and was thus impaired in polyunsaturated fatty acid biosynthesis was generated. The ¹²-desaturase mutant demonstrates delayed spore germination, a twofold reduction in growth, a reduced level of conidiation and complete loss of sclerotial development, compared to the wild-type. Host colonization is impaired, as reflected by a decrease in conidial production on live peanut and corn seed by the mutant compared to the wild-type. Similarly, the previously isolated A. nidulans ¹²-desaturase mutant has reduced colonization capabilities compared to the wild-type. Therefore, desaturation mutants display a key requisite that affords a genetic solution to oilseed crop contamination by mycotoxigenic Aspergillus species: a reduction in the production of conidia, the infectious particle of the pathogenic aspergilli.

The GenBank/EMBL/DDBJ accession number for the sequence reported in this paper is AF528822.

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