Microbiology 151 (2005), 421-431; DOI 10.1099/mic.0.27389-0
Microbiology 151 (2005), 421-431; DOI 10.1099/mic.0.27389-0
© 2005 Society for General Microbiology
Plasmid p256 from Lactobacillus plantarum represents a new type of replicon in lactic acid bacteria, and contains a toxin–antitoxin-like plasmid maintenance system
Elisabeth Sørvig1,2,
Morten Skaugen1,
Kristine Naterstad2,
Vincent G. H. Eijsink1 and
Lars Axelsson2
1 Department of Chemistry, Biotechnology and Food Science, Agricultural University of Norway, PO Box 5003, N-1432 Ås, Norway
2 Matforsk, Norwegian Food Research Institute, Osloveien 1, N-1430 Ås, Norway
Correspondence
Lars Axelsson
lars.axelsson{at}matforsk.no
Lactobacillus plantarum NC7 harbours a single 7·2 kb plasmid called p256. This report describes the complete nucleotide sequence and annotation of p256, as well as the identification of the minimal replicon of the plasmid. Based on sequence features in the unusually small (0·7 kb) minimal replicon, and the absence of a gene for a replication-relevant protein, p256 seems to represent a hitherto unknown type of theta replicon in lactic acid bacteria (LAB), with a relatively low copy-number. In addition, a putative toxin–antitoxin (TA) locus was identified. Experiments with variants of p256 indicated that the TA system was involved in plasmid maintenance. Furthermore, controlled expression of the TA genes stabilized vectors derived from the p256 replicon. To the authors' knowledge, this is the first time a TA locus with a demonstrated plasmid maintenance function has been identified in LAB. Transformation of several LAB with plasmids derived from p256 indicated that it has a narrow host range. Several effective expression vectors based on the p256 replicon have been constructed.
Abbreviations: CSP, cold-shock protein; LAB, lactic acid bacteria; RC, rolling circle; TA, toxin–antitoxin
The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences reported in this paper are AJ628941 (p256), AY557622 (pLPV111) and AY557620 (pELS100).
This article has been cited by other articles:
|
|
|
|
 
F. Fang, S. Flynn, Y. Li, M. J. Claesson, J.-P. van Pijkeren, J. K. Collins, D. van Sinderen, and P. W. O'Toole
Characterization of Endogenous Plasmids from Lactobacillus salivarius UCC118
Appl. Envir. Microbiol.,
May 15, 2008;
74(10):
3216 - 3228.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Broberg, K. Jacobsson, K. Strom, and J. Schnurer
Metabolite Profiles of Lactic Acid Bacteria in Grass Silage
Appl. Envir. Microbiol.,
September 1, 2007;
73(17):
5547 - 5552.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Criado, D. B. Diep, A. Aakra, J. Gutierrez, I. F. Nes, P. E. Hernandez, and L. M. Cintas
Complete Sequence of the Enterocin Q-Encoding Plasmid pCIZ2 from the Multiple Bacteriocin Producer Enterococcus faecium L50 and Genetic Characterization of Enterocin Q Production and Immunity.
Appl. Envir. Microbiol.,
October 1, 2006;
72(10):
6653 - 6666.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. van Belkum and M. E. Stiles
Characterization of the theta-type plasmid pCD3.4 from Carnobacterium divergens, and modulation of its host range by RepA mutation
Microbiology,
January 1, 2006;
152(1):
171 - 178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Sorvig, G. Mathiesen, K. Naterstad, V. G. H. Eijsink, and L. Axelsson
High-level, inducible gene expression in Lactobacillus sakei and Lactobacillus plantarum using versatile expression vectors
Microbiology,
July 1, 2005;
151(7):
2439 - 2449.
[Abstract]
[Full Text]
[PDF]
|
|
|
Copyright © 2005 Society for General Microbiology.
