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Microbiology 151 (2005), 749-755; DOI  10.1099/mic.0.27460-0
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Microbiology 151 (2005), 749-755; DOI  10.1099/mic.0.27460-0
© 2005 Society for General Microbiology

Skew-Laplace distribution in Gram-negative bacterial axenic cultures: new insights into intrinsic cellular heterogeneity

Olga Julià1 and Josep Vives-Rego2

1 Departament d'Estadística, Facultat de Matemàtiques, Universitat de Barcelona, Gran Via, 585, 08007-Barcelona, Spain
2 Departament de Microbiologia, Universitat de Barcelona, Av. Diagonal, 645, 08028-Barcelona, Spain

Correspondence
Josep Vives-Rego
jvives{at}ub.edu

The application of flow cytometry and skew-Laplace statistical analysis to assess cellular heterogeneity in Gram-negative axenic cultures is reported. In particular, fit to the log-skew-Laplace distribution for cellular side scatter or ‘granulosity’ is reported, and a number of theoretical and applied issues are considered in relation to the biological significance of this fit.


Abbreviations: FS, forward light scatter; SS, side light scatter







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