Microbiology 151 (2005), 1179-1186; DOI 10.1099/mic.0.27809-0
Microbiology 151 (2005), 1179-1186; DOI 10.1099/mic.0.27809-0
© 2005 Society for General Microbiology
ibeA, a virulence factor of avian pathogenic Escherichia coli
Pierre Germon1,
Yu-Hua Chen2,
Lina He2,
Jesús E. Blanco3,
Annie Brée1,
Catherine Schouler1,
Sheng-He Huang2 and
Maryvonne Moulin-Schouleur1
1 INRA – Centre de Tours, UR 86, Pathologie Bactérienne, 37380 Nouzilly, France
2 Children's Hospital Los Angeles and University of Southern California Keck School of Medicine, Los Angeles, CA 90027, USA
3 Laboratorio de Referencia de E. coli, Departamento de Microbiología y Parasitología, Faculdad de Veterinaria, Universidad de Santiago de Compostela, 27002 Lugo, Spain
Correspondence
Pierre Germon
germon{at}tours.inra.fr
The presence of ibeA, a gene encoding a known virulence factor of Escherichia coli strains responsible for neonatal meningitis in humans, was investigated in the genome of 213 avian pathogenic E. coli (APEC) strains and 55 non-pathogenic E. coli strains of avian origin. Fifty-three strains were found to be ibeA+, all of which belonged to the APEC group. The ibeA gene is therefore positively linked to the pathogenicity of strains (P<0·0001). Analysis of the serogroup of strains revealed a positive association of ibeA with serogroups O18, O88 and O2. On the contrary, only 1/59 O78 strains are ibeA+, indicating a negative association of ibeA with this serogroup (P<0·0001). The role of ibeA in the virulence of the APEC strain BEN 2908 was investigated by constructing an ibeA mutant. Challenge assays on 3-week-old chickens showed a reduced virulence for the ibeA mutant. Furthermore, the APEC strain BEN 2908 was able to invade brain microvascular epithelial cells, this invasion being significantly reduced upon inactivation of ibeA. Altogether, these results suggest a role of ibeA in the pathogenicity of some APEC strains and confirm the close relationship between APEC and other human extraintestinal pathogenic E. coli isolates.
Abbreviations: APEC, avian pathogenic Escherichia coli; BMEC, brain microvascular endothelial cells
The GenBank/EMBL/DDBJ accession number for the sequence reported in this paper is AY248744.
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Copyright © 2005 Society for General Microbiology.
