HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Coppi, M. V. Search for Related Content PubMed PubMed Citation Articles by Coppi, M. V. Agricola Articles by Coppi, M. V.

The hydrogenases of Geobacter sulfurreducens: a comparative genomic perspective

Department of Microbiology, 203N Morrill Science Center IVN, University of Massachusetts-Amherst, Amherst, MA 01003, USA

Correspondence
Maddalena V. Coppi
mcoppi{at}microbio.umass.edu

The hydrogenase content of the genome of Geobacter sulfurreducens, a member of the family Geobacteraceae within the -subdivision of the Proteobacteria, was examined and found to be distinct from that of Desulfovibrio species, another family of -Proteobacteria on which extensive research concerning hydrogen metabolism has been conducted. Four [NiFe]-hydrogenases are encoded in the G. sulfurreducens genome: two periplasmically oriented, membrane-bound hydrogenases, Hya and Hyb, and two cytoplasmic hydrogenases, Mvh and Hox. None of these [NiFe]-hydrogenases has a counterpart in Desulfovibrio species. Furthermore, the large and small subunits of Mvh and Hox appear to be related to archaeal and cyanobacterial hydrogenases, respectively. Clusters encoding [Fe]-hydrogenases and periplasmic [NiFeSe]-hydrogenases, which are commonly found in the genomes of Desulfovibrio species, are not present in the genome of G. sulfurreducens. Hydrogen-evolving Ech hydrogenases, which are present in the genomes of at least two Desulfovibrio species, were also absent from the G. sulfurreducens genome, despite the fact that G. sulfurreducens is capable of hydrogen production. Instead, the G. sulfurreducens genome contained a cluster encoding a multimeric Ech hydrogenase related (Ehr) complex that was similar in content to operons encoding Ech hydrogenases, but did not appear to encode a hydrogenase. Phylogenetic analysis revealed that the G. sulfurreducens ehr cluster is part of a family of related clusters found in both the Archaea and Bacteria.

This article has been cited by other articles:

				C. Risso, S. J. Van Dien, A. Orloff, D. R. Lovley, and M. V. Coppi Elucidation of an Alternate Isoleucine Biosynthesis Pathway in Geobacter sulfurreducens J. Bacteriol., April 1, 2008; 190(7): 2266 - 2274. [Abstract] [Full Text] [PDF]

				M. V. Coppi, R. A. O'Neil, C. Leang, F. Kaufmann, B. A. Methe, K. P. Nevin, T. L. Woodard, A. Liu, and D. R. Lovley Involvement of Geobacter sulfurreducens SfrAB in acetate metabolism rather than intracellular, respiration-linked Fe(III) citrate reduction Microbiology, October 1, 2007; 153(10): 3572 - 3585. [Abstract] [Full Text] [PDF]