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Microbiology 151 (2005), 1919-1926; DOI  10.1099/mic.0.27959-0
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Microbiology 151 (2005), 1919-1926; DOI  10.1099/mic.0.27959-0
© 2005 Society for General Microbiology

Oral immunization with a dam mutant of Yersinia pseudotuberculosis protects against plague

Victoria L. Taylor1, Richard W. Titball1,2 and Petra C. F. Oyston1

1 Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
2 Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel St, London WC1E 7HT, UK

Correspondence
Victoria L. Taylor
vltaylor{at}dstl.gov.uk

Inactivation of the gene encoding DNA adenine methylase (dam) has been shown to attenuate some pathogens such as Salmonella enterica serovar Typhimurium and is a lethal mutation in others such as Yersinia pseudotuberculosis strain YPIII. In this study the dam methylase gene in Yersinia pseudotuberculosis strain IP32953 was inactivated. Unlike the wild-type, DNA isolated from the mutant could be digested with MboI, which is consistent with an altered pattern of DNA methylation. The mutant was sensitive to bile salts but not to 2-aminopurine. The effect of dam inactivation on gene expression was examined using a DNA microarray. In BALB/c mice inoculated orally or intravenously with the dam mutant, the median lethal dose (MLD) was at least 106-fold higher than the MLD of the wild-type. BALB/c mice inoculated with the mutant were protected against a subcutaneous challenge with 100 MLDs of Yersinia pestis strain GB and an intravenous challenge with 300 MLDs of Y. pseudotuberculosis IP32953.

Abbreviations: 2-AP, 2-aminopurine; i.v., intravenous(ly); MLD, median lethal dose; s.c., subcutaneous(ly)




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