| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
2 Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel St, London WC1E 7HT, UK
Correspondence
Victoria L. Taylor
vltaylor{at}dstl.gov.uk
Inactivation of the gene encoding DNA adenine methylase (dam) has been shown to attenuate some pathogens such as Salmonella enterica serovar Typhimurium and is a lethal mutation in others such as Yersinia pseudotuberculosis strain YPIII. In this study the dam methylase gene in Yersinia pseudotuberculosis strain IP32953 was inactivated. Unlike the wild-type, DNA isolated from the mutant could be digested with MboI, which is consistent with an altered pattern of DNA methylation. The mutant was sensitive to bile salts but not to 2-aminopurine. The effect of dam inactivation on gene expression was examined using a DNA microarray. In BALB/c mice inoculated orally or intravenously with the dam mutant, the median lethal dose (MLD) was at least 106-fold higher than the MLD of the wild-type. BALB/c mice inoculated with the mutant were protected against a subcutaneous challenge with 100 MLDs of Yersinia pestis strain GB and an intravenous challenge with 300 MLDs of Y. pseudotuberculosis IP32953.
This article has been cited by other articles:
|
|
 |
|
  P. C. F. Oyston, M. A. Fox, S. J. Richards, and G. C. Clark Novel peptide therapeutics for treatment of infections J. Med. Microbiol., August 1, 2009; 58(8): 977 - 987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. L. Champion, I. A. M. Cooper, S. L. James, D. Ford, A. Karlyshev, B. W. Wren, M. Duffield, P. C. F. Oyston, and R. W. Titball Galleria mellonella as an alternative infection model for Yersinia pseudotuberculosis Microbiology, May 1, 2009; 155(5): 1516 - 1522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Pouillot, C. Fayolle, and E. Carniel Characterization of Chromosomal Regions Conserved in Yersinia pseudotuberculosis and Lost by Yersinia pestis Infect. Immun., October 1, 2008; 76(10): 4592 - 4599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Arlen, M. Singleton, J. J. Adamovicz, Y. Ding, A. Davoodi-Semiromi, and H. Daniell Effective Plague Vaccination via Oral Delivery of Plant Cells Expressing F1-V Antigens in Chloroplasts Infect. Immun., August 1, 2008; 76(8): 3640 - 3650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Blisnick, P. Ave, M. Huerre, E. Carniel, and C. E. Demeure Oral Vaccination against Bubonic Plague Using a Live Avirulent Yersinia pseudotuberculosis Strain Infect. Immun., August 1, 2008; 76(8): 3808 - 3816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Quenee, C. A. Cornelius, N. A. Ciletti, D. Elli, and O. Schneewind Yersinia pestis caf1 Variants and the Limits of Plague Vaccine Protection Infect. Immun., May 1, 2008; 76(5): 2025 - 2036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Falker, J. Schilling, M. A. Schmidt, and G. Heusipp Overproduction of DNA Adenine Methyltransferase Alters Motility, Invasion, and the Lipopolysaccharide O-Antigen Composition of Yersinia enterocolitica Infect. Immun., October 1, 2007; 75(10): 4990 - 4997. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Pouillot, C. Fayolle, and E. Carniel A putative DNA adenine methyltransferase is involved in Yersinia pseudotuberculosis pathogenicity Microbiology, August 1, 2007; 153(8): 2426 - 2434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Garbom, M. Olofsson, A.-C. Bjornfot, M. K. Srivastava, V. L. Robinson, P. C. F. Oyston, R. W. Titball, and H. Wolf-Watz Phenotypic characterization of a virulence-associated protein, VagH, of Yersinia pseudotuberculosis reveals a tight link between VagH and the type III secretion system Microbiology, May 1, 2007; 153(5): 1464 - 1473. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Erova, A. A. Fadl, J. Sha, B. K. Khajanchi, L. L. Pillai, E. V. Kozlova, and A. K. Chopra Mutations within the Catalytic Motif of DNA Adenine Methyltransferase (Dam) of Aeromonas hydrophila Cause the Virulence of the Dam-Overproducing Strain To Revert to That of the Wild-Type Phenotype. Infect. Immun., October 1, 2006; 74(10): 5763 - 5772. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Falker, M. A. Schmidt, and G. Heusipp Altered Ca2+ Regulation of Yop Secretion in Yersinia enterocolitica after DNA Adenine Methyltransferase Overproduction Is Mediated by Clp-Dependent Degradation of LcrG. J. Bacteriol., October 1, 2006; 188(20): 7072 - 7081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Casadesus and D. Low Epigenetic Gene Regulation in the Bacterial World Microbiol. Mol. Biol. Rev., September 1, 2006; 70(3): 830 - 856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Erova, L. Pillai, A. A. Fadl, J. Sha, S. Wang, C. L. Galindo, and A. K. Chopra DNA Adenine Methyltransferase Influences the Virulence of Aeromonas hydrophila Infect. Immun., January 1, 2006; 74(1): 410 - 424. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
