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Microbiology 151 (2005), 2393-2402; DOI  10.1099/mic.0.27953-0
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Microbiology 151 (2005), 2393-2402; DOI  10.1099/mic.0.27953-0
© 2005 Society for General Microbiology

Identification and targeted disruption of the gene encoding the main 3-ketosteroid dehydrogenase in Mycobacterium smegmatis

Anna Brzostek1, Tomasz Sliwinski2, Anna Rumijowska-Galewicz1, Malgorzata Korycka-Machala1 and Jaroslaw Dziadek1

1 Medical Biology Centre, Polish Academy of Sciences, Lodowa 106, 93-232 Lodz, Poland
2 Department of Biotechnology and Food Science, Technical University of Lodz, Wolczanska 171/173, 90-924 Lodz, Poland

Correspondence
Jaroslaw Dziadek
jdziadek{at}cbm.pan.pl

The catabolic potential for sterol degradation of fast-growing mycobacteria is well known. However, no genes or enzymes responsible for the steroid degradation process have been identified as yet in these species. One of the key enzymes required for degradation of the steroid ring structure is 3-ketosteroid {Delta}1-dehydrogenase (KsdD). The recent annotation of the Mycobacterium smegmatis genome (TIGR database) revealed six KsdD homologues. Targeted disruption of the MSMEG5898 (ksdD-1) gene, but not the MSMEG4855 (ksdD-2) gene, resulted in partial inactivation of the cholesterol degradation pathway and accumulation of the intermediate 4-androstene-3,17-dione. This effect was reversible by the introduction of the wild-type ksdD-1 gene into M. smegmatis {Delta}ksdD-1 or overexpression of ksdD-2. The data indicate that KsdD1 is the main KsdD in M. smegmatis, but that KsdD2 is able to perform the cholesterol degradation process when overproduced.

Abbreviations: 9OHAD, 9{alpha}-hydroxy-4-androstene-3,17-dione; AD, 4-androstene-3,17-dione; ADD, 1,4-androstadiene-3,17-dione; DCO, double-crossover homologous recombinant; Kan, kanamycin; KsdD, 3-ketosteroid {Delta}1-dehydrogenase; SCO, single-crossover homologous recombinant




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 Appl. Environ. Microbiol.Home page
A. Andor, A. Jekkel, D. A. Hopwood, F. Jeanplong, E. Ilkoy, A. Konya, I. Kurucz, and G. Ambrus
Generation of Useful Insertionally Blocked Sterol Degradation Pathway Mutants of Fast-Growing Mycobacteria and Cloning, Characterization, and Expression of the Terminal Oxygenase of the 3-Ketosteroid 9{alpha}-Hydroxylase in Mycobacterium smegmatis mc2155.
Appl. Envir. Microbiol., October 1, 2006; 72(10): 6554 - 6559.
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