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Cell lysis directed by ^E in early stationary phase and effect of induction of the rpoE gene on global gene expression in Escherichia coli

¹ Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan
² CREST, Nara Institute of Science and Technology, Nara 630-01, Japan
³ R&E Center of Informatics, Nara Institute of Science and Technology, Nara 630-01, Japan
⁴ Graduate School of Information Science, Nara Institute of Science and Technology, Nara 630-01, Japan
⁵ Laboratory of Plasma Membrane and Nuclear Signalling, Graduate School of Biostudies, 1302, Kyoto University, Kyoto 606-8502, Japan

Correspondence
Mamoru Yamada
m-yamada{at}yamaguchi-u.ac.jp

It has been shown that Escherichia coli cells with increased expression of the rpoE gene encoding ^E exhibit enhanced cell lysis in early stationary phase. Further analysis of the lysis phenomenon was performed using a transient expression system of the rpoE gene and by DNA microarray. The former analysis revealed a ^E-directed cell lysis, specific for early stationary phase but not for the exponential phase. The microarray analysis with RNAs from exponential and early stationary phase cells revealed that a large number of genes were up- or down-regulated when the rpoE gene was induced, and that several genes were induced in a phase-specific manner. The upregulated genes include many previously identified ^E regulon genes, suggesting that a large number of genes are under the control of ^E in this organism. These genes are involved in various cellular activities, including the cell envelope, cellular processes, regulatory functions, transport and translation. Genes that are presumably related to phase-specific cell lysis in E. coli are discussed.

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