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Microbiology 152 (2006), 3529-3534; DOI  10.1099/mic.0.29276-0
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Microbiology 152 (2006), 3529-3534; DOI  10.1099/mic.0.29276-0
© 2006 Society for General Microbiology

Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans

Igor Kucera

Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, CZ-62500 Brno, Czech Republic

Correspondence
Igor Kucera
ikucera{at}chemi.muni.cz

When grown anaerobically on a succinate+nitrate (SN) medium, Paracoccus denitrificans forms the membrane-bound, cytoplasmically oriented, chlorate-reducing nitrate reductase Nar, while the periplasmic enzyme Nap is expressed during aerobic growth on butyrate+oxygen (BO) medium. Preincubation of SN cells with chlorate produced a concentration-dependent decrease in nitrate utilization, which could be ascribed to Nar inactivation. Toluenization rendered Nar less sensitive to chlorate, but more sensitive to chlorite, suggesting that the latter compound may be the true inactivator. The Nap enzyme of BO cells was inactivated by both chlorate and chlorite at concentrations that were at least two orders of magnitude lower than those shown to affect Nar. Partial purification of Nap resulted in insensitivity to chlorate and diminished sensitivity to chlorite. Azide was specific for SN cells in protecting nitrate reductase against chlorate attack, the protective effect of nitrate being more pronounced in BO cells. The results are discussed in terms of different metabolic activation of chlorine oxoanions in both types of cells, and limited permeation of chlorite across the cell membrane.

Abbreviations: BO, butyrate+oxygen; MV, methyl viologen; SN, succinate+nitrate






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Copyright © 2006 Society for General Microbiology.