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Microbiology 152 (2006), 3561-3568; DOI  10.1099/mic.0.29227-0
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Microbiology 152 (2006), 3561-3568; DOI  10.1099/mic.0.29227-0
© 2006 Society for General Microbiology

Growth-phase-dependent mobility of the lvh-encoding region in Legionella pneumophila strain Paris

Anne Doléans-Jordheim1, Mongi Akermi1, Christophe Ginevra1, Christel Cazalet2, Elizabeth Kay3, Dominique Schneider3, Carmen Buchrieser2, Danièle Atlan4, François Vandenesch1, Jerome Etienne1 and Sophie Jarraud1

1 INSERM, E0230, Lyon, F-69008 France; Université Lyon 1, Centre National de référence des Legionella, Faculté Laennec, Lyon, F-69008 France
2 Unité de Génomique des Micro-organismes Pathogènes, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
3 Laboratoire Adaptation et Pathogénie des Micro-organismes, CNRS UMR5163, Université Joseph Fourier-Institut Jean Roget, 38700 La Tronche, France
4 Unité de Microbiologie et Génétique UMR 5122, Université Claude Bernard Lyon 1, Bât. Lwoff, 10 rue Dubois, 69622 Villeurbanne, France

Correspondence
Sophie Jarraud
sophie.jarraud{at}univ-lyon1.fr

The lvh region of the Legionella pneumophila genome, which encodes a type IV secretion system, is located on a plasmid-like element in strains Paris (pP36) and Philadelphia (pLP45). The pP36 element has been described either integrated in the chromosome or excised as a multi-copy plasmid, in a similar manner to pLP45. In this paper, the chromosomal integration of pP36 in the Paris strain genome was described, occurring through site-specific recombination at the 3' end of a transfer-messenger RNA gene by recombination between attachment sites, in a similar manner to pathogenicity islands. This integration was growth-phase dependent, occurring during the exponential phase. Several pP36-borne genes were expressed during the lag phase of bacterial growth, coinciding with the peak amount of the episomal form of pP36. Expression of the same genes decreased during the exponential and stationary phases, owing to the integration phenomenon and a loss of episomal copies of pP36. A similar plasmid-like element was described in the Lens strain genome, suggesting that the mobility of the lvh region is a phenomenon widespread among Legionella sp.

Abbreviations: Ct, cycle threshold; Tm, melting curve peak; tmRNA, transfer-messenger RNA




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