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Microbiology 152 (2006), 529-534; DOI  10.1099/mic.0.28431-0
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Microbiology 152 (2006), 529-534; DOI  10.1099/mic.0.28431-0
© 2006 Society for General Microbiology

The structural proteome of Pseudomonas aeruginosa bacteriophage {phi}KMV

Rob Lavigne1, Jean-Paul Noben2, Kirsten Hertveldt1, Pieter-Jan Ceyssens1, Yves Briers1, Debora Dumont2, Bart Roucourt1, Victor N. Krylov3, Vadim V. Mesyanzhinov1,4, Johan Robben2 and Guido Volckaert1

1 Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium
2 Biomedical Research Institute, Limburgs Universitair Centrum and School of Life Sciences, University Hasselt, Diepenbeek, B-3590, Belgium
3 State Institute for Genetics and Selection of Industrial Micro-organisms, 1st Dorozhnii proezd 1, Moscow, 113545 Russia
4 Howard Hughes Medical Institute; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Street 16/10, Moscow, 117991 Russia

Correspondence
Rob Lavigne
rob.lavigne{at}biw.kuleuven.be

The structural proteome of {phi}KMV, a lytic bacteriophage infecting Pseudomonas aeruginosa, was analysed using two approaches. In one approach, structural proteins of the phage were fractionated by SDS-PAGE for identification by liquid chromatography-mass spectrometry (LC-MS). In a second approach, a whole-phage shotgun analysis (WSA) was applied. WSA uses trypsin digestion of whole phage particles, followed by reversed-phase HPLC and gas-phase fractionation of the complex peptide mixture prior to MS. The results yield a comprehensive view of structure-related proteins in {phi}KMV and suggest subtle structural differences from phage T7.

Abbreviations: WSA, whole-phage shotgun analysis

The identification analyses of the proteins (Mascot and Lutefisk files) can be verified in the supplementary table of data available with the online version of this paper.




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