|
|
||||||||
) associated with a virulent lineage of serotype III Streptococcus agalactiae
1 Department of Biology, James Madison University, Harrisonburg, VA 22807, USA
2 Department of Infectious Diseases, St Jude Children's Research Hospital, Memphis, TN 38105, USA
3 Department of Pediatrics, University of Utah Health Sciences Center, Salt Lake City, UT 84132, USA
4 Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL 32610, USA
Correspondence
L. Jeannine Brady
jbrady{at}dental.ufl.edu
Group B streptococci (GBS) are pathogens of both neonates and adults, with serotype III strains in particular being associated with invasive disease and meningitis. In this study, a novel GBS surface antigen,
, was found to be co-expressed with the previously reported
antigen on an identical subset of serotype III GBS. Expression of
/
on the surface of serotype III GBS was shown to distinguish the restriction digest pattern (RDP) III-3 and multilocus sequence typing (ST)-17 lineage.
-Specific antibodies were reactive with a unique, high-molecular-mass, serine-rich repeat protein (Srr-2) found exclusively in RDP III-3 strains. The gene encoding Srr-2 was located within a putative accessory secretory locus that included secY2 and secA2 homologues and had a genetic organization similar to that of the secY2/A2 locus of staphylococci. In contrast, serotype III
/
-negative strains and strains representative of serotypes Ia, Ib, Ic and II shared a common Srr-encoding gene, srr-1, and an organization of the secY2/A2 locus similar to that of previously reported serotype Ic,
/
-negative serotype III and serotype V GBS strains. Representative serotype III
/
-positive strains had LD90 values 34 logs less than those of serotype III
/
-negative strains in a neonatal mouse model of infection. These results indicate that the RDP III-3/ST-17 lineage expresses Srr-2 and is highly virulent in an in vivo model of neonatal sepsis.
The GenBank/EMBL/DDBJ accession numbers for the sequences of the secY2/A2 loci from GBS strains 874391 and J48 reported in this paper are AY669067 and DQ174691, respectively.
This article has been cited by other articles:
![]() |
S. Bu, Y. Li, M. Zhou, P. Azadin, M. Zeng, P. Fives-Taylor, and H. Wu Interaction between Two Putative Glycosyltransferases Is Required for Glycosylation of a Serine-Rich Streptococcal Adhesin J. Bacteriol., February 15, 2008; 190(4): 1256 - 1266. [Abstract] [Full Text] [PDF] |
||||
![]() |
U. Samen, B. J. Eikmanns, D. J. Reinscheid, and F. Borges The Surface Protein Srr-1 of Streptococcus agalactiae Binds Human Keratin 4 and Promotes Adherence to Epithelial HEp-2 Cells Infect. Immun., November 1, 2007; 75(11): 5405 - 5414. [Abstract] [Full Text] [PDF] |
||||
![]() |
Q. Chen, B. Sun, H. Wu, Z. Peng, and P. M. Fives-Taylor Differential Roles of Individual Domains in Selection of Secretion Route of a Streptococcus parasanguinis Serine-Rich Adhesin, Fap1 J. Bacteriol., November 1, 2007; 189(21): 7610 - 7617. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. A. Bensing, I. R. Siboo, and P. M. Sullam Glycine Residues in the Hydrophobic Core of the GspB Signal Sequence Route Export toward the Accessory Sec Pathway J. Bacteriol., May 15, 2007; 189(10): 3846 - 3854. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. Wu, M. Zeng, and P. Fives-Taylor The Glycan Moieties and the N-Terminal Polypeptide Backbone of a Fimbria-Associated Adhesin, Fap1, Play Distinct Roles in the Biofilm Development of Streptococcus parasanguinis Infect. Immun., May 1, 2007; 75(5): 2181 - 2188. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. Wu, S. Bu, P. Newell, Q. Chen, and P. Fives-Taylor Two Gene Determinants Are Differentially Involved in the Biogenesis of Fap1 Precursors in Streptococcus parasanguis J. Bacteriol., February 15, 2007; 189(4): 1390 - 1398. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |