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Microbiology 152 (2006), 1109-1118; DOI  10.1099/mic.0.28376-0
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Microbiology 152 (2006), 1109-1118; DOI  10.1099/mic.0.28376-0
© 2006 Society for General Microbiology

Identification of the Acidobacterium capsulatum LexA box reveals a lateral acquisition of the Alphaproteobacteria lexA gene

Gerard Mazón1, Susana Campoy1, Ivan Erill2 and Jordi Barbé1,3

1 Centre de Recerca en Sanitat Animal (CReSA), Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
2 Biomedical Applications Group, Centro Nacional de Microelectrónica, 08193 Bellaterra, Spain
3 Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain

Correspondence
Jordi Barbé
jordi.barbe{at}uab.es

Acidobacterium capsulatum is the most thoroughly studied species of a new bacterial phylogenetic group designated the phylum Acidobacteria. Through a TBLASTN search, the A. capsulatum lexA gene has been identified, and its product purified. Electrophoretic mobility shift assays have shown that A. capsulatum LexA protein binds specifically to the direct repeat GTTCN7GTTC motif. Strikingly, this is also the LexA box of the Alphaproteobacteria, but had not previously been described outside this subclass of the Proteobacteria. In addition, a phylogenetic analysis of the LexA protein clusters together Acidobacterium and the Alphaproteobacteria, moving the latter away from their established phylogenetic position as a subclass of the Proteobacteria, and pointing to a lateral gene transfer of the lexA gene from the phylum Acidobacteria, or an immediate ancestor, to the Alphaproteobacteria. Lastly, in vivo experiments demonstrate that the A. capsulatum recA gene is DNA-damage inducible, despite the fact that a LexA-binding sequence is not present in its promoter region.

Abbreviations: EMSA, electrophoretic mobility shift assay; LGT, lateral gene transfer




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