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Microbiology 152 (2006), 1169-1177; DOI  10.1099/mic.0.28703-0
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Microbiology 152 (2006), 1169-1177; DOI  10.1099/mic.0.28703-0
© 2006 Society for General Microbiology

Genes encoding ribonucleoside hydrolase 1 and 2 from Corynebacterium ammoniagenes

Hyun-Soo Kim2,{dagger}, Jin-Ho Lee2,{dagger}, Won-Sik Lee2 and Won-Gi Bang1

1 Department of Biotechnology and Genetic Engineering, College of Life and Environmental Sciences, Korea University, Anam-dong, Seoul 136-701, Korea
2 R&D Center for Bioproducts, CJ Corp., Seoul 157-724, Korea

Correspondence
Won-Gi Bang
agrchem{at}korea.ac.kr

Two kinds of nucleoside hydrolases (NHs) encoded by rih1 and rih2 were cloned from Corynebacterium ammoniagenes using deoD- and gsk-defective Escherichia coli. Sequence analysis revealed that NH 1 was a protein of 337 aa with a deduced molecular mass of 35 892 Da, whereas NH 2 consisted of 308 aa with a calculated molecular mass of 32 310 Da. Experiments with crude extracts of IPTG-induced E. coli CGSC 6885(pTNU23) and 6885(pTNI12) indicated that the Rih1 enzyme could catalyse the hydrolysis of uridine and cytidine and showed pyrimidine-specific ribonucleoside hydrolase activity. Rih2 was able to hydrolyse both purine and pyrimidine ribonucleosides with the following order of activity – inosine>adenosine>uridine>guanosine>xanthosine>cytidine – and was classified in the non-specific NHs family. rih1 and rih2 deletion mutants displayed a decrease in cell growth on minimal medium supplemented with pyrimidine and purine/pyrimidine nucleosides, respectively, compared with the wild-type strain. Growth of each mutant was substantially complemented by introducing rih1 and rih2, respectively. Furthermore, disruption of both rih1 and rih2 led to the inability of the mutant to utilize purine and pyrimidine nucleosides as sole carbon source on minimal medium. These results indicated that rih1 and rih2 play major roles in the salvage pathways of nucleosides in this micro-organism.

Abbreviations: NH, nucleoside hydrolase

{dagger}These authors contributed equally to this work.

The GenBank/EMBL/DDBJ accession numbers for the sequences of rih1 and rih2 reported in this paper are AY603363 and AY603360, respectively.




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