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Identification of the ^E regulon of Salmonella enterica serovar Typhimurium

¹ Institute of Molecular Biology, Centre of Excellence for Molecular Medicine, Slovak Academy of Science, Dubravska cesta 21, 845 51 Bratislava, Slovak Republic
² Molecular Bacteriology Group, Institute of Comparative Medicine, Department of Veterinary Pathology, Glasgow University Veterinary School, Bearsden Road, Glasgow G61 1QH, UK

Correspondence
Jan Kormanec
jan.kormanec{at}savba.sk

The extracytoplasmic function sigma factor, ^E, has been shown to play a critical role in virulence of Salmonella enterica serovar Typhimurium (S. Typhimurium). The previously optimized two-plasmid system has been used to identify S. Typhimurium promoters recognized by RNA polymerase containing ^E. This method allowed identification of 34 ^E-dependent promoters that direct expression of 62 genes in S. Typhimurium, 23 of which (including several specific for S. Typhimurium) have not been identified previously to be dependent upon ^E in Escherichia coli. The promoters were confirmed in S. Typhimurium and transcriptional start points of the promoters were determined by S1-nuclease mapping. All the promoters contained sequences highly similar to the consensus sequence of ^E-dependent promoters. The identified genes belonging to the S. Typhimurium ^E-regulon encode proteins involved in primary metabolism, DNA repair systems and outer-membrane biogenesis, and regulatory proteins, periplasmic proteases and folding factors, proposed lipoproteins, and inner- and outer-membrane proteins with unknown functions. Several of these ^E-dependent genes have been shown to play a role in virulence of S. Typhimurium.

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