Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 152 (2006), 1601-1607; DOI  10.1099/mic.0.28658-0
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by O'Connell, C. M.
Right arrow Articles by Nicks, K. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by O'Connell, C. M.
Right arrow Articles by Nicks, K. M.
Agricola
Right arrow Articles by O'Connell, C. M.
Right arrow Articles by Nicks, K. M.
Microbiology 152 (2006), 1601-1607; DOI  10.1099/mic.0.28658-0
© 2006 Society for General Microbiology

A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture

Catherine M. O'Connell and Kristy M. Nicks

Department of Microbiology and Immunology, UAMS, 4301 West Markham, Little Rock, AR 72205, USA

Correspondence
Catherine M. O'Connell
oconnellcatherine{at}uams.edu

A highly conserved cryptic plasmid is present in Chlamydia trachomatis yet naturally occurring plasmid-deficient isolates are very rare. This paper describes the isolation and characterization of a plasmid-deficient strain of C. muridarum, using novobiocin as a curing agent. Plasmid-deficient derivatives of C. muridarum strain Nigg were generated at high efficiencies (4–30 %). Phenotypic characterization revealed that the cured derivative was unable to accumulate glycogen within intracytoplasmic inclusions. In addition, this strain formed small plaques at a reduced efficiency compared to the wild-type parent.

Abbreviations: i.f.u., inclusion-forming units




This article has been cited by other articles:


Home page
 Infect. Immun.Home page
Z. Li, D. Chen, Y. Zhong, S. Wang, and G. Zhong
The Chlamydial Plasmid-Encoded Protein pgp3 Is Secreted into the Cytosol of Chlamydia-Infected Cells
Infect. Immun., August 1, 2008; 76(8): 3415 - 3428.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. H. Carlson, W. M. Whitmire, D. D. Crane, L. Wicke, K. Virtaneva, D. E. Sturdevant, J. J. Kupko III, S. F. Porcella, N. Martinez-Orengo, R. A. Heinzen, et al.
The Chlamydia trachomatis Plasmid Is a Transcriptional Regulator of Chromosomal Genes and a Virulence Factor
Infect. Immun., June 1, 2008; 76(6): 2273 - 2283.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
R. DeMars and J. Weinfurter
Interstrain Gene Transfer in Chlamydia trachomatis In Vitro: Mechanism and Significance
J. Bacteriol., March 1, 2008; 190(5): 1605 - 1614.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. M. O'Connell, R. R. Ingalls, C. W. Andrews Jr., A. M. Scurlock, and T. Darville
Plasmid-Deficient Chlamydia muridarum Fail to Induce Immune Pathology and Protect against Oviduct Disease
J. Immunol., September 15, 2007; 179(6): 4027 - 4034.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2006 Society for General Microbiology.