HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Pham, V. D. Articles by Singer, M. Search for Related Content PubMed PubMed Citation Articles by Pham, V. D. Articles by Singer, M. Agricola Articles by Pham, V. D. Articles by Singer, M.

The response regulator PhoP4 is required for late developmental events in Myxococcus xanthus

Vinh D. Pham^1,

Conrad W. Shebelut^1,

¹ Section of Microbiology and Center for Genetics and Development, 268 Briggs Hall, University of California, Davis, CA 95616, USA
² Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK

Correspondence
Mitchell Singer
mhsinger{at}ucdavis.edu

Phosphate regulation is complex in the developmental prokaryote Myxococcus xanthus, and requires at least four two-component systems (TCSs). Here, the identification and characterization of a member of one TCS, designated PhoP4, is reported. phoP4 insertion and in-frame deletion strains caused spore viability to be decreased by nearly two orders of magnitude, and reduced all three development-specific phosphatase activities by 80–90 % under phosphate-limiting conditions. Microarray and quantitative PCR analyses demonstrated that PhoP4 is also required for appropriate expression of the predicted pstSCAB–phoU operon of inorganic phosphate assimilation genes. Unlike the case for the other three M. xanthus Pho TCSs, the chromosomal region around phoP4 does not contain a partner histidine kinase gene. Yeast two-hybrid analyses reveal that PhoP4 interacts reciprocally with PhoR2, the histidine kinase of the Pho2 TCS; however, the existence of certain phenotypic differences between phoP4 and phoR2 mutants suggests that PhoP4 interacts with another, as-yet unidentified, histidine kinase.

Present address: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Present address: Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.

This article has been cited by other articles:

				D. E. Whitworth, A. B. Holmes, A. G. Irvine, D. A. Hodgson, and D. J. Scanlan Phosphate Acquisition Components of the Myxococcus xanthus Pho Regulon Are Regulated by both Phosphate Availability and Development J. Bacteriol., March 15, 2008; 190(6): 1997 - 2003. [Abstract] [Full Text] [PDF]