| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Pathobiology, 2522 VMBSB, 2001 S. Lincoln Avenue, University of Illinois, Urbana, IL 61802, USA
2 Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA
Correspondence
Lois L. Hoyer
lhoyer{at}uiuc.edu
Candida albicans ALS3 encodes a large cell-surface glycoprotein that has adhesive properties. Immunostaining of cultured C. albicans germ tubes showed that Als3p is distributed diffusely across the germ tube surface. Two-photon laser scanning microscopy of model catheter biofilms grown using a PALS3-green fluorescent protein (GFP) reporter strain showed GFP production in hyphae throughout the biofilm structure while biofilms grown using a PTPI1-GFP reporter strain showed GFP in both hyphae and yeast-form cells. Model catheter biofilms formed by an als3
/als3 strain were weakened structurally and had approximately half the biomass of a wild-type biofilm. Reintegration of a wild-type ALS3 allele restored biofilm mass and wild-type biofilm structure. Production of an Als3p–Ag
1p fusion protein under control of the ALS3 promoter in the als3/als3 strain restored some of the wild-type biofilm structural features, but not the wild-type biofilm mass. Despite its inability to restore wild-type biofilm mass, the Als3p–Ag1p fusion protein mediated adhesion of the als3/als3 C. albicans strain to human buccal epithelial cells (BECs). The adhesive role of the Als3p N-terminal domain was further demonstrated by blocking adhesion of C. albicans to BECs with immunoglobulin reactive against the Als3p N-terminal sequences. Together, these data suggest that portions of Als3p that are important for biofilm formation may be different from those that are important in BEC adhesion, and that Als3p may have multiple functions in biofilm formation. Overexpression of ALS3 in an efg1/efg1 strain that was deficient for filamentous growth and biofilm formation resulted in growth of elongated C. albicans cells, even under culture conditions that do not favour filamentation. In the catheter biofilm model, the ALS3 overexpression strain formed biofilm with a mass similar to that of a wild-type control. However, C. albicans cells in the biofilm had yeast-like morphology. This result uncouples the effect of cellular morphology from biofilm formation and underscores the importance of Als3p in biofilm development on silicone elastomer surfaces.
This article has been cited by other articles:
|
|
 |
|
  C. Stichternoth and J. F. Ernst Hypoxic Adaptation by Efg1 Regulates Biofilm Formation by Candida albicans Appl. Envir. Microbiol., June 1, 2009; 75(11): 3663 - 3672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. MacCallum, L. Castillo, K. Nather, C. A. Munro, A. J. P. Brown, N. A. R. Gow, and F. C. Odds Property Differences among the Four Major Candida albicans Strain Clades Eukaryot. Cell, March 1, 2009; 8(3): 373 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. L. Chaffin Candida albicans Cell Wall Proteins Microbiol. Mol. Biol. Rev., September 1, 2008; 72(3): 495 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. N. Otoo, K. G. Lee, W. Qiu, and P. N. Lipke Candida albicans Als Adhesins Have Conserved Amyloid-Forming Sequences Eukaryot. Cell, May 1, 2008; 7(5): 776 - 782. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Goyard, P. Knechtle, M. Chauvel, A. Mallet, M.-C. Prevost, C. Proux, J.-Y. Coppee, P. Schwartz, F. Dromer, H. Park, et al. The Yak1 Kinase Is Involved in the Initiation and Maintenance of Hyphal Growth in Candida albicans Mol. Biol. Cell, May 1, 2008; 19(5): 2251 - 2266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. Sosinska, P. W. J. de Groot, M. J. Teixeira de Mattos, H. L. Dekker, C. G. de Koster, K. J. Hellingwerf, and F. M. Klis Hypoxic conditions and iron restriction affect the cell-wall proteome of Candida albicans grown under vagina-simulative conditions Microbiology, February 1, 2008; 154(2): 510 - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Norice, F. J. Smith Jr., N. Solis, S. G. Filler, and A. P. Mitchell Requirement for Candida albicans Sun41 in Biofilm Formation and Virulence Eukaryot. Cell, November 1, 2007; 6(11): 2046 - 2055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Yeater, J. Chandra, G. Cheng, P. K. Mukherjee, X. Zhao, S. L. Rodriguez-Zas, K. E. Kwast, M. A. Ghannoum, and L. L. Hoyer Temporal analysis of Candida albicans gene expression during biofilm development Microbiology, August 1, 2007; 153(8): 2373 - 2385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhao, S.-H. Oh, and L. L. Hoyer Unequal contribution of ALS9 alleles to adhesion between Candida albicans and human vascular endothelial cells Microbiology, July 1, 2007; 153(7): 2342 - 2350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Dranginis, J. M. Rauceo, J. E. Coronado, and P. N. Lipke A Biochemical Guide to Yeast Adhesins: Glycoproteins for Social and Antisocial Occasions Microbiol. Mol. Biol. Rev., June 1, 2007; 71(2): 282 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Biswas, P. Van Dijck, and A. Datta Environmental Sensing and Signal Transduction Pathways Regulating Morphopathogenic Determinants of Candida albicans Microbiol. Mol. Biol. Rev., June 1, 2007; 71(2): 348 - 376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Li, M. J. Svarovsky, A. J. Karlsson, J. P. Wagner, K. Marchillo, P. Oshel, D. Andes, and S. P. Palecek Eap1p, an Adhesin That Mediates Candida albicans Biofilm Formation In Vitro and In Vivo Eukaryot. Cell, June 1, 2007; 6(6): 931 - 939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Argimon, J. A. Wishart, R. Leng, S. Macaskill, A. Mavor, T. Alexandris, S. Nicholls, A. W. Knight, B. Enjalbert, R. Walmsley, et al. Developmental Regulation of an Adhesin Gene during Cellular Morphogenesis in the Fungal Pathogen Candida albicans Eukaryot. Cell, April 1, 2007; 6(4): 682 - 692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Richard and A. Plaine Comprehensive Analysis of Glycosylphosphatidylinositol-Anchored Proteins in Candida albicans Eukaryot. Cell, February 1, 2007; 6(2): 119 - 133. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
