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Morphological changes and proteome response of Corynebacterium glutamicum to a partial depletion of FtsI

Angelina Ramos^1,

¹ Departamento de Ecología, Genética y Microbiología, Área de Microbiología, Facultad de Biología, Universidad de León, 24071 León, Spain
² Centro Biología Molecular ‘Severo Ochoa’, Consejo Superior de Investigaciones Científicas, CSIC-UAM, Campus Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain
³ Institut fur Genomforschung, Universitat Bielefeld, Universitatsstrasse 25, D-33615 Bielefeld, Germany

Correspondence
José A. Gil
degjgs{at}unileon.es

In Corynebacterium glutamicum, as in many Gram-positive bacteria, the cell division gene ftsI is located at the beginning of the dcw cluster, which comprises cell division- and cell wall-related genes. Transcriptional analysis of the cluster revealed that ftsI is transcribed as part of a polycistronic mRNA, which includes at least mraZ, mraW, ftsL, ftsI and murE, from a promoter that is located upstream of mraZ. ftsI appears also to be expressed from a minor promoter that is located in the intergenic ftsL–ftsI region. It is an essential gene in C. glutamicum, and a reduced expression of ftsI leads to the formation of larger and filamentous cells. A translational GFP-FtsI fusion protein was found to be functional and localized to the mid-cell of a growing bacterium, providing evidence of its role in cell division in C. glutamicum. This study involving proteomic analysis (using 2D SDS-PAGE) of a C. glutamicum strain that has partially depleted levels of FtsI reveals that at least 20 different proteins were overexpressed in the organism. Eight of these overexpressed proteins, which include DivIVA, were identified by MALDI-TOF. Overexpression of DivIVA was confirmed by Western blotting using anti-DivIVA antibodies, and also by fluorescence microscopy analysis of a C. glutamicum RESF1 strain expressing a chromosomal copy of a divIVA-gfp transcriptional fusion. Overexpression of DivIVA was not observed when FtsI was inhibited by cephalexin treatment or by partial depletion of FtsZ.

Present Address: Departamento de Biología Funcional, Área de Microbiología, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain.

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				M. Letek, E. Ordonez, J. Vaquera, W. Margolin, K. Flardh, L. M. Mateos, and J. A. Gil DivIVA Is Required for Polar Growth in the MreB-Lacking Rod-Shaped Actinomycete Corynebacterium glutamicum J. Bacteriol., May 1, 2008; 190(9): 3283 - 3292. [Abstract] [Full Text] [PDF]