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Microbiology 153 (2007), 16-28; DOI  10.1099/mic.0.29211-0
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Microbiology 153 (2007), 16-28; DOI  10.1099/mic.0.29211-0
© 2007 Society for General Microbiology

An rhl-like quorum-sensing system negatively regulates pyoluteorin production in Pseudomonas sp. M18

An Yan, Xianqing Huang, Haiming Liu, Dexian Dong, Dabing Zhang, Xuehong Zhang and Yuquan Xu

Key Laboratory of Microbial Metabolism, Ministry of Education, College of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China

Correspondence
Yuquan Xu
xuyq{at}sjtu.edu.cn

Pseudomonas sp. M18, isolated from the watermelon rhizosphere, is antagonistic against a number of soil-borne pathogens. This strain produces an uncharacterized red pigment, pyoluteorin (Plt), and two N-acylhomoserine lactones (AHLs). A previously isolated red-pigment-defective mutant, M18-T510, contains an insert within a gene similar to rhlI in P. aeruginosa PAO1. The M18 rhlI gene product is responsible for the production of two AHL signals: N-butyryl-homoserine lactone and N-hexanoylhomoserine lactone. Mutants defective in either rhlI or rhlR showed enhanced Plt biosynthesis due to loss of transcriptional repression, which was mediated, at least in part, by suppressed expression of the activator PltR. A Plt-specific ABC transporter was also upregulated in the rhl mutants in a Plt-dependent manner. In comparison with the wild-type strain, the rhl mutants survived longer during stationary-phase growth.

Abbreviations: AHL, N-acylhomoserine lactone; BHL, N-butyrylhomoserine lactone; CT, threshold cycle; HHL, N-hexanoylhomoserine lactone; PCA, phenazine-1-carboxylic acid; Plt, pyoluteorin; QS, quorum sensing

The GenBank/EMBL/DDBJ accession number for the nucleotide sequence of the rhlI gene and the partial sequence of the rhlR gene of Pseudomonas sp. M18 is DQ345445.






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Copyright © 2007 Society for General Microbiology.