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Src homology domain 2 adaptors affect adherence of Salmonella enterica serovar Typhimurium to non-phagocytic cells

¹ Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada
² Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z4, Canada
³ Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC V6T 1Z4, Canada
⁴ Cell Biology Program, Hospital for Sick Children, Toronto, ON M5G 1X8, Canada
⁵ Department of Molecular and Medical Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada

Correspondence
B. Brett Finlay
bfinlay{at}interchange.ubc.ca

The ability of Salmonella enterica serovar Typhimurium (S. Typhimurium) to penetrate the intestinal epithelium is key to its pathogenesis. Bacterial invasion can be seen as a two-step process initially requiring adherence to the host cell surface followed by internalization into the host cell. Evidence suggests that adherence of S. Typhimurium to host cells is receptor-mediated; however, the host cell receptor(s) has/have not been identified. Internalization of S. Typhimurium absolutely requires the actin cytoskeleton yet only a few of the cytoskeletal components involved in this process have been identified. In order to identify host proteins that may play a role in S. Typhimurium invasion, the recruitment of actin-associated proteins was investigated. The contribution of recruited Src homology 2 adaptor proteins to invasion was further investigated and it was found that, while not involved in bacterial internalization itself, the adaptors Nck and ShcA influenced adherence of S. Typhimurium to non-phagocytic cells.

A supplementary figure showing recruitment of actin-associated proteins to sites of S. Typhimurium invasion, a supplementary table listing host proteins investigated for their recruitment to sites of S. Typhimurium invasion, and details of the methods used to produce this data are available with the online version of this paper.

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