HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary table Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Marr, A. K. Articles by Hancock, R. E. W. Search for Related Content PubMed PubMed Citation Articles by Marr, A. K. Articles by Hancock, R. E. W. Agricola Articles by Marr, A. K. Articles by Hancock, R. E. W.

The Lon protease of Pseudomonas aeruginosa is induced by aminoglycosides and is involved in biofilm formation and motility

Alexandra K. Marr

Centre for Microbial Diseases and Immunity Research, University of British Columbia, 2259 Lower Mall, Vancouver, British Columbia V6T 1Z4, Canada

Correspondence
Robert E. W. Hancock
bob{at}cmdr.ubc.ca

Pseudomonas aeruginosa is an important nosocomial opportunistic human pathogen and a major cause of chronic lung infections in individuals with cystic fibrosis. Serious infections by this organism are often treated with a combination of aminoglycosides and semi-synthetic penicillins. Subinhibitory concentrations of antibiotics are now being recognized for their role in microbial persistence and the development of antimicrobial resistance, two very important clinical phenomena. An extensive screen of a P. aeruginosa PAO1 luciferase gene fusion library was performed to identify genes that were differentially regulated during exposure to subinhibitory gentamicin. It was demonstrated that subinhibitory concentrations of gentamicin and tobramycin induced a set of genes that are likely to affect the interaction of P. aeruginosa with host cells, including the gene encoding Lon protease, which is known to play a major role in protein quality control. Studies with a lon mutant compared to its parent and a complemented strain indicated that this protein was essential for biofilm formation and motility in P. aeruginosa.

Present address: Department of Zoology, University of British Columbia, 6270 University Boulevard, Vancouver, British Columbia V6T 1Z4, Canada.

This article has been cited by other articles:

				W. J. Gooderham, M. Bains, J. B. McPhee, I. Wiegand, and R. E. W. Hancock Induction by Cationic Antimicrobial Peptides and Involvement in Intrinsic Polymyxin and Antimicrobial Peptide Resistance, Biofilm Formation, and Swarming Motility of PsrA in Pseudomonas aeruginosa J. Bacteriol., August 15, 2008; 190(16): 5624 - 5634. [Abstract] [Full Text] [PDF]

				R. P. Ryan and J. M. Dow Diffusible signals and interspecies communication in bacteria Microbiology, July 1, 2008; 154(7): 1845 - 1858. [Abstract] [Full Text] [PDF]

				J. B. Goldberg, R. E. W. Hancock, R. E. Parales, J. Loper, and P. Cornelis Pseudomonas 2007 J. Bacteriol., April 15, 2008; 190(8): 2649 - 2662. [Full Text] [PDF]

				J. Overhage, M. Bains, M. D. Brazas, and R. E. W. Hancock Swarming of Pseudomonas aeruginosa Is a Complex Adaptation Leading to Increased Production of Virulence Factors and Antibiotic Resistance J. Bacteriol., April 15, 2008; 190(8): 2671 - 2679. [Abstract] [Full Text] [PDF]

				M. D. Brazas, E. B. M. Breidenstein, J. Overhage, and R. E. W. Hancock Role of Lon, an ATP-Dependent Protease Homolog, in Resistance of Pseudomonas aeruginosa to Ciprofloxacin Antimicrob. Agents Chemother., December 1, 2007; 51(12): 4276 - 4283. [Abstract] [Full Text] [PDF]

				J. Overhage, S. Lewenza, A. K. Marr, and R. E. W. Hancock Identification of Genes Involved in Swarming Motility Using a Pseudomonas aeruginosa PAO1 Mini-Tn5-lux Mutant Library J. Bacteriol., March 1, 2007; 189(5): 2164 - 2169. [Abstract] [Full Text] [PDF]