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Microbiology 153 (2007), 760-767; DOI  10.1099/mic.0.2006/000372-0
© 2007 Society for General Microbiology

Proteomic analysis of a non-virulent mutant of the phytopathogenic bacterium Erwinia chrysanthemi deficient in osmoregulated periplasmic glucans: change in protein expression is not restricted to the envelope, but affects general metabolism

Franck Bouchart{dagger}, Aurélie Delangle{dagger}, Jérôme Lemoine, Jean-Pierre Bohin and Jean-Marie Lacroix

Unité de Glycobiologie Structurale et Fonctionnelle, UMR USTL-CNRS 8576 IFR 118, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq Cedex, France

Correspondence
Jean-Marie Lacroix
jean-marie.lacroix{at}univ-lille1.fr

Osmoregulated periplasmic glucans (OPGs) are general constituents of the envelope of Gram-negative bacteria. They are required for full virulence of bacterial phytopathogens such as Pseudomonas syringae, Xanthomonas campestris and Erwinia chrysanthemi. E. chrysanthemi is a pectinolytic {gamma}-proteobacterium that causes soft rot disease on a wide range of plant species. In addition to the loss of virulence, opg mutants exhibit a pleiotropic phenotype that affects motility, bile-salt resistance, exoenzyme secretion, exopolysaccharide synthesis and membrane lipid composition. This is believed to be the first proteomic analysis of an OPG-defective mutant of E. chrysanthemi and it revealed that, in addition to the effects described, catabolic enzyme synthesis was enhanced and there was a greater abundance of some proteins catalysing the folding and degradation of proteins needed for various stress responses. Thus, in the opg mutant strain, loss of virulence was the result of a combination of envelope structure changes and cellular metabolism modifications.

Abbreviations: OPG, osmoregulated periplasmic glucans; TCA, tricarboxylic acid

{dagger}These authors contributed equally to this work.




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