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Microbiology 153 (2007), 1149-1156; DOI  10.1099/mic.0.2006/003905-0
© 2007 Society for General Microbiology

Extracellular carbonic anhydrases of the stromatolite-forming cyanobacterium Microcoleus chthonoplastes

Elena Kupriyanova1, Arsenio Villarejo2,3, Alexandra Markelova1, Lyudmila Gerasimenko4, Georgy Zavarzin4, Göran Samuelsson2, Dmitry A. Los1 and Natalia Pronina1

1 Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276 Russia
2 Umeå Plant Science Centre, Department of Plant Physiology, University of Umeå, S-901 87 Umeå, Sweden
3 Department of Biology, Universidad Autonoma de Madrid, 28049 Madrid
4 Institute of Microbiology, Russian Academy of Sciences, Prospect 60-Letiya Oktyabrya 7/2, Moscow, 117312 Russia

Correspondence
Natalia Pronina
pronina{at}ippras.ru

Active extracellular carbonic anhydrases (CAs) were found in the alkaliphilic stromatolite-forming cyanobacterium Microcoleus chthonoplastes. Enzyme activity was detected in intact cells and in the cell envelope fraction. Western blot analysis of polypeptides from the cell envelope suggested the presence of at least two polypeptides cross-reacting with antibodies against both {alpha} and beta classes of CA. Immunocytochemical analysis revealed putative {alpha}-CA localized in the glycocalyx. This {alpha}-CA has a molecular mass of about 34 kDa and a pI of 3.5. External CAs showed two peaks of activity at around pH 10 and 7.5. The possible involvement of extracellular CAs of M. chthonoplastes in photosynthetic assimilation of inorganic carbon and its relationship to CaCO3 deposition during mineralization of cyanobacterial cells are discussed.

Abbreviations: CA, carbonic anhydrase; Ci, inorganic carbon; WAU, Wilbur–Anderson units






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