Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 153 (2007), 1221-1228; DOI  10.1099/mic.0.2006/002758-0
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Giacomodonato, M. N.
Right arrow Articles by Cerquetti, M. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Giacomodonato, M. N.
Right arrow Articles by Cerquetti, M. C.
Agricola
Right arrow Articles by Giacomodonato, M. N.
Right arrow Articles by Cerquetti, M. C.
Microbiology 153 (2007), 1221-1228; DOI  10.1099/mic.0.2006/002758-0
© 2007 Society for General Microbiology

SipA, SopA, SopB, SopD and SopE2 effector proteins of Salmonella enterica serovar Typhimurium are synthesized at late stages of infection in mice

M. N. Giacomodonato1, S. Uzzau2, D. Bacciu2, R. Caccuri1, S. H. Sarnacki1, S. Rubino2 and M. C. Cerquetti1

1 Centro de Estudios Farmacológicos y Botánicos CEFyBO-CONICET, Universidad de Buenos Aires, Facultad de Medicina, Departamento de Microbiología, Parasitología e Immunología, Buenos Aires, Argentina
2 Dipartimento di Scienze Biomediche, Università di Sassari, Italy

Correspondence
M. N. Giacomodonato
monicagiaco{at}yahoo.com.ar

Salmonella pathogenicity island (SPI)-1 is essential for invasion of non-phagocytic cells, whereas SPI-2 is required for intracellular survival and proliferation in phagocytes. Some SPI-1 effectors, however, are induced upon invasion of both phagocytic and non-phagocytic cells, suggesting that they may also be required post-invasion. In the present work, the presence was analysed of SipA, SopA, SopB, SopD and SopE2 effector proteins of Salmonella enterica serovar Typhimurium in vitro and in vivo during murine salmonellosis. Tagged (3xFLAG) strains of S. enterica serovar Typhimurium were inoculated intraperitoneally or intragastrically to BALB/c mice and recovered from the spleen and mesenteric lymph nodes of moribund mice. Tagged proteins were detected by SDS-PAGE and immunoblotting with anti-FLAG antibodies. In vitro experiments showed that SPI-1 effector proteins SipA, SopA, SopB, SopD and SopE2 were secreted under SPI-1 conditions. Interestingly, it was found that S. enterica serovar Typhimurium continued to synthesize SipA, SopB, SopD and SopE2 in colonized organs for several days, regardless of the route of inoculation. Together, these results indicate that SPI-1 effector proteins may participate in the late stages of Salmonella infection in mice.

Abbreviations: i.g., intragastric(ally); iNOS, inducible nitric oxide synthase; i.p., intraperitoneal(ly); MLN, mesenteric lymph node; serovar Typhimurium, Salmonella enterica serovar Typhimurium; SPI, Salmonella pathogenicity island; TTSS, type III secretion system




This article has been cited by other articles:


Home page
 Infect. Immun.Home page
T. S. Desin, P.-K. S. Lam, B. Koch, C. Mickael, E. Berberov, A. L. S. Wisner, H. G. G. Townsend, A. A. Potter, and W. Koster
Salmonella enterica Serovar Enteritidis Pathogenicity Island 1 Is Not Essential for but Facilitates Rapid Systemic Spread in Chickens
Infect. Immun., July 1, 2009; 77(7): 2866 - 2875.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
I. Martinez-Argudo and M. A. Jepson
Salmonella translocates across an in vitro M cell model independently of SPI-1 and SPI-2
Microbiology, December 1, 2008; 154(12): 3887 - 3894.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. H. Bustamante, L. C. Martinez, F. J. Santana, L. A. Knodler, O. Steele-Mortimer, and J. L. Puente
HilD-mediated transcriptional cross-talk between SPI-1 and SPI-2
PNAS, September 23, 2008; 105(38): 14591 - 14596.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2007 Society for General Microbiology.