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The key role of the mycolic acid content in the functionality of the cell wall permeability barrier in Corynebacterineae

¹ Institut für Biochemie der Universität zu Köln, Zülpicher Straße 47, 50674 Köln, Germany
² Départment ‘Mécanismes Moléculaires des Infections Mycobactériennes’, Institut de Pharmacologie et Biologie Structurale (UMR 5089 du Centre National de la Recherche Scientifique et de l'Université Paul Sabatier), 205 route de Narbonne, 31077 Toulouse cedex 04, France

Correspondence
Susanne Morbach
s.morbach{at}uni-koeln.de

Recently, it has been shown that trehalose and mycolic acids are essential for the growth of Mycobacterium tuberculosis, the causative agent of tuberculosis, and Mycobacterium smegmatis, and important but not indispensable to the survival of Corynebacterium glutamicum. Therefore, to investigate the function of mycolic acids in both the permeability of the cell wall to small nutrients and antibiotics, and the excretion of amino acids by C. glutamicum, a trehalose-deficient mutant of the L-lysine producer ATCC 21527, designated LPtreSotsAtreY, was constructed. By using different carbon sources in either the presence or the absence of external trehalose, a set of endogenously trehalose-free LPtreSotsAtreY cells that exhibited various mycolate contents was generated. The results showed that the structure of the arabinogalactan of these different cell types of LPtreSotsAtreY was not affected when the mycolic acid layer was either missing or impaired. Nevertheless, cells were more susceptible to antibiotics, and the permeability of their cell walls to glycerol was increased. Interestingly, a concomitant increase in the excretion of both L-lysine and L-glutamate was also observed, indicating that the mycolic acid content of the permeability barrier (and not only the peptidoglycan and/or the arabinogalactan) is implicated in the glutamate excretion process.

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