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-Butyrolactone autoregulator-receptor system involved in lankacidin and lankamycin production and morphological differentiation in Streptomyces rochei

Department of Molecular Biotechnology, Hiroshima University, Higashi-Hiroshima 739-8530, Japan

Correspondence
Haruyasu Kinashi
kinashi{at}hiroshima-u.ac.jp

An afsA homologue (srrX) and three -butyrolactone receptor gene homologues (srrA, srrB and srrC) are coded on the giant linear plasmid pSLA2-L in Streptomyces rochei 7434AN4, a producer of two polyketide antibiotics, lankacidin and lankamycin. Construction of gene disruptants and their phenotypic study revealed that srrX and srrA make a -butyrolactone receptor system in this strain. Addition of a -butyrolactone fraction to an srrX-deficient mutant restored the production of lankacidin and lankamycin, indicating that the SrrX protein is not necessary for this event. In addition to a positive effect on antibiotic production, srrX showed a negative effect on morphological differentiation. The receptor gene srrA reversed both effects of srrX, while the second receptor gene homologue srrC had only a positive function in spore formation. Furthermore, disruption of the third homologue srrB greatly increased the production of lankacidin and lankamycin. Electron microscopic analysis showed that aerial mycelium formation stopped at a different stage in the srrA and srrC mutants. Overall, these results indicated that srrX, srrA, srrB and srrC constitute a complex regulatory system for antibiotic production and morphological differentiation in S. rochei.

A table of strains and plasmids, and details of the disruption of srrX, srrA, srrB and srrC, are available as supplementary data with the online version of this paper.

This article has been cited by other articles:

				S. Yamamoto, Y. He, K. Arakawa, and H. Kinashi {gamma}-Butyrolactone-Dependent Expression of the Streptomyces Antibiotic Regulatory Protein Gene srrY Plays a Central Role in the Regulatory Cascade Leading to Lankacidin and Lankamycin Production in Streptomyces rochei J. Bacteriol., February 15, 2008; 190(4): 1308 - 1316. [Abstract] [Full Text] [PDF]