| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Division of Microbiology, Institute for Animal Health, Compton, Berkshire RG20 7NN, UK
2 Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK
Correspondence
Mark P. Stevens
mark-p.stevens{at}bbsrc.ac.uk
Salmonella enterica serovar Typhimurium (S. Typhimurium) is a zoonotic enteric pathogen of worldwide importance and pigs are a significant reservoir of human infection. Signature-tagged transposon mutagenesis (STM) was used to identify genes required by S. Typhimurium to colonize porcine intestines. A library of 1045 signature-tagged mutants of S. Typhimurium ST4/74 NalR was screened following oral inoculation of pigs in duplicate. A total of 119 attenuating mutations were identified in 95 different genes, many of which encode known or putative secreted or surface-anchored molecules. A large number of attenuating mutations were located within Salmonella pathogenicity islands (SPI)-1 and -2, confirming important roles for type III secretion systems (T3SS)-1 and -2 in intestinal colonization of pigs. Roles for genes encoded in other pathogenicity islands and islets, including the SPI-6-encoded Saf atypical fimbriae, were also identified. Given the role of secreted factors and the protection conferred against other pathogens by vaccination with extracellular and type III secreted proteins, the efficacy of a secreted protein vaccine from wild-type S. Typhimurium following intramuscular vaccination of pigs was evaluated. Serum IgG responses against type III secreted proteins were induced following vaccination and a significant reduction in faecal excretion of S. Typhimurium was observed in the acute phase of infection compared to mock-vaccinated animals. Vaccination with secreted proteins from an isogenic S. Typhimurium prgH mutant produced comparable levels of protection to vaccination with the preparation from the parent strain, indicating that protection was not reliant on T3SS-1 secreted proteins. The data provide valuable information for the control of Salmonella in pigs.
Present address: Ridgeway Biologicals Ltd, c/o Institute for Animal Health, Compton, Newbury RG20 7NN, UK.
This article has been cited by other articles:
|
|
 |
|
  M. P. Stevens, T. J. Humphrey, and D. J. Maskell Molecular insights into farm animal and zoonotic Salmonella infections Phil Trans R Soc B, September 27, 2009; 364(1530): 2709 - 2723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. D. Pullinger, F. Dziva, B. Charleston, T. S. Wallis, and M. P. Stevens Identification of Salmonella enterica Serovar Dublin-Specific Sequences by Subtractive Hybridization and Analysis of Their Role in Intestinal Colonization and Systemic Translocation in Cattle Infect. Immun., November 1, 2008; 76(11): 5310 - 5321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Huang, C. L. Leming, M. Suyemoto, and C. Altier Genome-Wide Screen of Salmonella Genes Expressed during Infection in Pigs, Using In Vivo Expression Technology Appl. Envir. Microbiol., December 1, 2007; 73(23): 7522 - 7530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. D. Pullinger, S. M. Paulin, B. Charleston, P. R. Watson, A. J. Bowen, F. Dziva, E. Morgan, B. Villarreal-Ramos, T. S. Wallis, and M. P. Stevens Systemic Translocation of Salmonella enterica Serovar Dublin in Cattle Occurs Predominantly via Efferent Lymphatics in a Cell-Free Niche and Requires Type III Secretion System 1 (T3SS-1) but Not T3SS-2 Infect. Immun., November 1, 2007; 75(11): 5191 - 5199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Paulin, A. Jagannathan, J. Campbell, T. S. Wallis, and M. P. Stevens Net Replication of Salmonella enterica Serovars Typhimurium and Choleraesuis in Porcine Intestinal Mucosa and Nodes Is Associated with Their Differential Virulence Infect. Immun., August 1, 2007; 75(8): 3950 - 3960. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
