| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Key Laboratory of Medical Molecular Virology of Ministry of Education and Public Health, Institutes of Biomedical Sciences and Medical Microbiology, Shanghai Medical School of Fudan University Box 228, Yi Xue Yuan Road 138#, Shanghai 200032, P. R. China
2 BioCentrum-DTU, Building 301, Technical University of Denmark, DK-2800 Lyngby, Denmark
Correspondence
Soeren Molin
sm{at}biocentrum.dtu.dk
Staphylococcus epidermidis has become a serious nosocomial pathogen frequently causing infections associated with implanted foreign materials. Biofilm formation is considered a major factor determining S. epidermidis pathogenicity in such device-associated infections. Here, evidence is presented that extracellular DNA is important for the initial phase of biofilm development by S. epidermidis on polystyrene or glass surfaces under static or hydrodynamic conditions. Comparative PCR amplification from S. epidermidis chromosomal and extracellular DNA indicated that the extracellular DNA is similar to chromosomal DNA. Experiments involving the S. epidermidis wild-type and an isogenic atlE mutant indicated that most of the extracellular DNA in S. epidermidis cultures and biofilms is generated through activity of the autolysin AtlE. The presented results suggest that extracellular DNA is generated in S. epidermidis populations through AtlE-mediated lysis of a subpopulation of the bacteria, and that the extracellular DNA promotes biofilm formation of the remaining population.
These authors contributed equally to this work.
This article has been cited by other articles:
|
|
 |
|
  E. Karatan and P. Watnick Signals, Regulatory Networks, and Materials That Build and Break Bacterial Biofilms Microbiol. Mol. Biol. Rev., June 1, 2009; 73(2): 310 - 347. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Vilain, J. M. Pretorius, J. Theron, and V. S. Brozel DNA as an Adhesin: Bacillus cereus Requires Extracellular DNA To Form Biofilms Appl. Envir. Microbiol., May 1, 2009; 75(9): 2861 - 2868. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. G. A. Fredheim, C. Klingenberg, H. Rohde, S. Frankenberger, P. Gaustad, T. Flaegstad, and J. E. Sollid Biofilm Formation by Staphylococcus haemolyticus J. Clin. Microbiol., April 1, 2009; 47(4): 1172 - 1180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ganeshnarayan, S. M. Shah, M. R. Libera, A. Santostefano, and J. B. Kaplan Poly-N-Acetylglucosamine Matrix Polysaccharide Impedes Fluid Convection and Transport of the Cationic Surfactant Cetylpyridinium Chloride through Bacterial Biofilms Appl. Envir. Microbiol., March 1, 2009; 75(5): 1308 - 1314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. T. Luong, M. G. Lei, and C. Y. Lee Staphylococcus aureus Rbf Activates Biofilm Formation In Vitro and Promotes Virulence in a Murine Foreign Body Infection Model Infect. Immun., January 1, 2009; 77(1): 335 - 340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gazzola and P. S. Cocconcelli Vancomycin heteroresistance and biofilm formation in Staphylococcus epidermidis from food Microbiology, October 1, 2008; 154(10): 3224 - 3231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. C. Thomas, L. R. Thurlow, D. Boyle, and L. E. Hancock Regulation of Autolysis-Dependent Extracellular DNA Release by Enterococcus faecalis Extracellular Proteases Influences Biofilm Development J. Bacteriol., August 15, 2008; 190(16): 5690 - 5698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kreth, Y. Zhang, and M. C. Herzberg Streptococcal Antagonism in Oral Biofilms: Streptococcus sanguinis and Streptococcus gordonii Interference with Streptococcus mutans J. Bacteriol., July 1, 2008; 190(13): 4632 - 4640. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Seidl, C. Goerke, C. Wolz, D. Mack, B. Berger-Bachi, and M. Bischoff Staphylococcus aureus CcpA Affects Biofilm Formation Infect. Immun., May 1, 2008; 76(5): 2044 - 2050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Izano, M. A. Amarante, W. B. Kher, and J. B. Kaplan Differential Roles of Poly-N-Acetylglucosamine Surface Polysaccharide and Extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis Biofilms Appl. Envir. Microbiol., January 15, 2008; 74(2): 470 - 476. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
