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Genome sequence comparison and superinfection between two related Pseudomonas aeruginosa phages, D3112 and MP22

Yun-Jeong Heo^1,

In-Young Chung^1,

¹ Department of Life Science, Sogang University, Seoul 121-742, Korea
² Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL 61802, USA

Correspondence
You-Hee Cho
youhee{at}sogang.ac.kr

A temperate transposable bacteriophage (MP22) was isolated from a Korean clinical isolate of Pseudomonas aeruginosa. It has a coliphage -like morphology and a double-stranded DNA genome. The complete nucleotide sequence and annotation of the MP22 genome and its characteristics are presented. The MP22 genome is 36 409 bp long with a G+C content of 64.2 mol%. The genome contains 51 proposed ORFs, of which 48 (94 %) display synteny and significant nucleotide and protein sequence similarity to the corresponding ORFs of the closely related phage, D3112. Three of the predicted ORFs are unique proteins, whose functions are yet to be revealed. The phage c repressors exhibit striking dissimilarities and, when present as a single gene, did not show cross-immunity. In contrast, although an MP22 lysogen could be productively infected with D3112, MP22 could not grow on a D3112 lysogen, indicating a role of other D3112 genes in superinfection exclusion.

These authors contributed equally to this work.

The GenBank/EMBL/DDBJ accession number for the complete genome sequence of MP22 is DQ873690.

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