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Involvement of BmoR and BmoG in n-alkane metabolism in ‘Pseudomonas butanovora’

¹ Department of Botany and Plant Pathology, 2082 Cordley Hall, Oregon State University, Corvallis, OR 97331, USA
² Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA
³ Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331, USA

Correspondence
Luis A. Sayavedra-Soto
sayavedl{at}science.oregonstate.edu

‘Pseudomonas butanovora’ uses an alcohol-inducible alkane monooxygenase (BMO) to grow on C₂–C₉ n-alkanes. Five ORFs were identified flanking the BMO structural genes. Two of the ORFs, bmoR, encoding a putative ⁵⁴-transcriptional regulator BmoR, and bmoG, encoding a putative GroEL chaperonin BmoG, were analysed by gene-inactivation experiments. The BmoR-deficient mutant grew at slower growth rates than the wild-type on C₂–C₅ n-alkanes and showed little to no growth on C₆–C₈ n-alkanes within 7 days. A BmoR-deficient mutant was constructed in the ‘P. butanovora’ bmoX : : lacZ reporter strain and used to test whether bmoR was involved in bmoX induction after growth on C₂–C₈ carbon sources. In acetate- or lactate-grown cells, C₂–C₈ n-alcohols failed to induce β-galactosidase activity. In contrast, in propionate-, butyrate- or pentanoate-grown cells, n-butanol induced 45 % of the β-galactosidase activity observed in the control bmoX : : lacZ strain. In propionate-grown cells, C₂–C₅ n-alcohols induced β-galactosidase activity, whereas C₇ and C₈ n-alcohols did not. BmoR may act as a ⁵⁴-transcriptional regulator of bmo that is controlled by the n-alcohol produced in the alkane oxidation. During growth on short-chain-length fatty acids, however, another BMO regulatory system seems to be activated to promote transcription of bmo by short-chain-length alcohols (i.e. C₆). The bmoG-deficient mutant did not grow on C₂–C₈ n-alkanes; however, it was capable of transcribing bmoX and bmoC of the BMO operon. BmoG may act as a chaperonin to assemble competent BMO.

The GenBank/EMBL/DDBJ accession number for the sequence of the bmo operon and its adjacent genes of ‘Pseudomonas butanovora’ is AY093933.

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				D. M. Doughty, E. G. Kurth, L. A. Sayavedra-Soto, D. J. Arp, and P. J. Bottomley Evidence for Involvement of Copper Ions and Redox State in Regulation of Butane Monooxygenase in Pseudomonas butanovora J. Bacteriol., April 15, 2008; 190(8): 2933 - 2938. [Abstract] [Full Text] [PDF]