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Revisiting the role of yeast Sfp1 in ribosome biogenesis and cell size control: a chemostat study

Chiara Cipollina^1,

¹ Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca, P.za della Scienza 2, 20126 Milano, Italy
² Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands
³ Kluyver Centre for Genomics of Industrial Fermentation, Julianalaan 67, 2628 BC Delft, The Netherlands

Correspondence
Johannes H. de Winde
j.h.dewinde{at}tudelft.nl

Saccharomyces cerevisiae SFP1 promotes transcription of a large cluster of genes involved in ribosome biogenesis. During growth in shake flasks, a mutant deleted for SFP1 shows a small size phenotype and a reduced growth rate. We characterized the behaviour of an sfp1 mutant compared to an isogenic reference strain growing in chemostat cultures at the same specific growth rate. By studying glucose (anaerobic)- and ethanol (aerobic)-limited cultures we focused specifically on nutrient-dependent effects. Major differences in the genome-wide transcriptional profiles were observed during glucose-limited growth. In particular, Sfp1 appeared to be involved in the control of ribosome biogenesis but not of ribosomal protein gene expression. Flow cytometric analyses revealed size defects for the mutant under both growth conditions. Our results suggest that Sfp1 plays a role in transcriptional and cell size control, operating at two different levels of the regulatory network linking growth, metabolism and cell size.

Present address: Chiara Cipollina, Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.

The Gene Expression Omnibus accession number for the data reported in this paper is GSE5238.

Three tables listing genes showing significantly changed expression in the sfp1 null mutant compared to the reference strain during ethanol-limited and glucose-limited growth, and expression levels of the ribosomal protein genes, are available with the online version of this paper.

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