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Microbiology 154 (2008), 3073-3083; DOI  10.1099/mic.0.2008/019828-0
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Microbiology 154 (2008), 3073-3083; DOI  10.1099/mic.0.2008/019828-0
© 2008 Society for General Microbiology

Effect of carbon source availability and growth phase on expression of Corynebacterium glutamicum genes involved in the tricarboxylic acid cycle and glyoxylate bypass

Sung Ok Han1,2, Masayuki Inui1 and Hideaki Yukawa1

1 Molecular Microbiology and Biotechnology Group, Research Institute of Innovative Technology for the Earth (RITE), Kyoto 619-0292, Japan
2 College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea

Correspondence
Hideaki Yukawa
mmg-lab{at}rite.or.jp
Sung Ok Han
samhan{at}korea.ac.kr

The effect of different carbon sources on the expression of tricarboxylic acid (TCA) cycle genes, along with glyoxylate bypass genes, in Corynebacterium glutamicum was determined. All TCA cycle genes were coordinately expressed in medium containing acetate. Growth in the presence of acetate gave rise to abundant expression of most TCA cycle genes, with the level of gltA transcript being the highest. However, when the cells entered the stationary phase triggered by acetate exhaustion, all genes were repressed, except sucCD and mdhB, which were slightly induced. Acetate withdrawal from the growth medium during the exponential phase also led to rapid repression of most TCA cycle genes and a corresponding twofold increase in the expression of sucCD, which were strongly induced by citrate and succinate. In addition, glucose depletion during the stationary phase led to a corresponding 8–20-fold induction of the sucCD, aceA and aceB genes. Addition of glucose to acetate medium resulted in about 10-fold induction of sucCD. The strong dependence of TCA cycle sucCD and glyoxylate bypass aceA and aceB expression on carbon source availability was confirmed and the regulatory system will be studied precisely.

Abbreviations: CBS, CRP-binding site; CCR, carbon catabolite repression; CRP, cAMP receptor protein; DO, dissolved oxygen; EMSA, electrophoretic mobility shift assay; ORP, oxidation-reduction potentials; qRT-PCR, quantitative real-time reverse transcription PCR; RLM-RACE, RNA ligase-mediated rapid amplification of cDNA ends; TCA cycle, tricarboxylic acid cycle






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