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Microbiology 154 (2008), 550-558; DOI  10.1099/mic.0.2007/010868-0IMMEDIATE OPEN ACCESS ARTICLE
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Microbiology 154 (2008), 550-558; DOI  10.1099/mic.0.2007/010868-0
© 2008 Society for General Microbiology

Identification of the Staphylococcus aureus MSCRAMM clumping factor B (ClfB) binding site in the {alpha}C-domain of human fibrinogen

Evelyn J. Walsh1, Helen Miajlovic1, Oleg V. Gorkun2 and Timothy J. Foster1

1 Microbiology Department, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland
2 Department of Pathology and Laboratory Medicine, CB #7525, Brinkhous-Bullitt Building, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7525, USA

Correspondence
Timothy J. Foster
tfoster{at}tcd.ie

Clumping factor B (ClfB) of Staphylococcus aureus binds to cytokeratin 10 and to fibrinogen. In this study the binding site in human fibrinogen was localized to a short region within the C terminus of the A{alpha}-chain. ClfB only bound to the A{alpha}-chain of fibrinogen in a ligand-affinity blot and in solid-phase assays with purified recombinant fibrinogen chains. A variant of fibrinogen with wild-type Bβ- and {gamma}-chains but with a deletion that lacked the C-terminal residues from 252–610 of the A{alpha}-chain did not support adherence of S. aureus Newman expressing ClfB. A series of truncated mutants of the recombinant A{alpha}-chain were tested for their ability to support adherence of S. aureus Newman ClfB+, which allowed the binding site to be localized to a short segment of the unfolded flexible repeated sequence within the C terminus of the A{alpha}-chain. This was confirmed by two amino acid substititions within repeat 5 of the recombinant A{alpha}-chain which did not support adherence of Newman ClfB+. Lactococcus lactis expressing ClfB mutants with amino acid substitutions (N256 and Q235) located in the putative ligand-binding trench between domains N2 and N3 of the A-domain were defective in adherence to immobilized fibrinogen and cytokeratin 10, suggesting that both ligands bind to the same or overlapping regions.

Abbreviations: CK10, cytokeratin 10; Fg, fibrinogen; HRP, horseradish peroxidase; MSCRAMMs, microbial surface components recognizing adhesive matrix molecules






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