HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary data Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bronowski, C. Articles by Winstanley, C. Search for Related Content PubMed PubMed Citation Articles by Bronowski, C. Articles by Winstanley, C. Agricola Articles by Bronowski, C. Articles by Winstanley, C.

A subset of mucosa-associated Escherichia coli isolates from patients with colon cancer, but not Crohn's disease, share pathogenicity islands with urinary pathogenic E. coli

¹ Division of Medical Microbiology, School of Infection and Host Defence, University of Liverpool, Daulby Street, Liverpool L69 3GA, UK
² Division of Gastroenterology, School of Clinical Science, University of Liverpool, Crown Street, Liverpool L69 3BX, UK

Correspondence
Craig Winstanley
C.Winstanley{at}liv.ac.uk

Adherent and invasive mucosa-associated Escherichia coli have been implicated in the pathogenesis of colon cancer and inflammatory bowel diseases. It has been reported that such isolates share features of extraintestinal E. coli (ExPEC) and particularly uropathogenic E. coli (UPEC). We used suppression subtractive hybridization (SSH) to subtract the genome of E. coli K-12 from that of a colon cancer mucosal E. coli isolate. Of the subtracted sequences, 53 % were present in the genomes of one or more of three sequenced UPEC strains but absent from the genome of an enterohaemorrhagic E. coli (EHEC) strain. Of the subtracted sequences, 80 % matched at least one UPEC genome, whereas only 4 % were absent from the UPEC genomes but present in the genome of the EHEC strain. A further genomic subtraction against the UPEC strain 536 enriched for sequences matching mobile genetic elements, other ExPEC strains, and other UPEC strains or commensals, rather than strains associated with gastrointestinal disease. We analysed the distribution of selected subtracted sequences and UPEC-associated pathogenicity islands (PAIs) amongst a panel of mucosa-associated E. coli isolated from colonoscopic biopsies of patients with colon cancer, patients with Crohn's disease and controls. This enabled us to identify a group of isolates from colon cancer (30–40 %) carrying multiple genes previously categorized as UPEC-specific and implicated in virulence.

Supplementary tables showing the E. coli isolates used in this study and distribution of sequences according to PCR assays, the oligonucleotide primers used for PCR amplification, and the percentage distribution of PAIs amongst E. coli collections are available with the online version of this paper.

This article has been cited by other articles:

				C. N Bernstein and F. Shanahan Disorders of a modern lifestyle: reconciling the epidemiology of inflammatory bowel diseases Gut, September 1, 2008; 57(9): 1185 - 1191. [Full Text] [PDF]