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Microbiology 154 (2008), 1281-1289; DOI  10.1099/mic.0.2007/015610-0
© 2008 Society for General Microbiology

A global modulatory role for the Yersinia enterocolitica H-NS protein

Rosa C. Baños1, José I. Pons2, Cristina Madrid2 and Antonio Juárez1,2

1 Institut de Bioenginyeria de Catalunya, Parc Científic de Barcelona, Edifici Hèlix. c/ Josep Samitier 1-5, 08028 Barcelona, Spain
2 Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, Avda Diagonal 645, 08028 Barcelona, Spain

Correspondence
Antonio Juárez
ajuarez{at}ub.edu

The H-NS protein plays a significant role in the modulation of gene expression in Gram-negative bacteria. Whereas isolation and characterization of hns mutants in Escherichia coli, Salmonella and Shigella represented critical steps to gain insight into the modulatory role of H-NS, it has hitherto not been possible to isolate hns mutants in Yersinia. The hns mutation is considered to be deleterious in this genus. To study the modulatory role of H-NS in Yersinia we circumvented hns lethality by expressing in Y. enterocolitica a truncated H-NS protein known to exhibit anti-H-NS activity in E. coli (H-NSTEPEC). Y. enterocolitica cells expressing H-NSTEPEC showed an altered growth rate and several differences in the protein expression pattern, including the ProV protein, which is modulated by H-NS in other enteric bacteria. To further confirm that H-NSTEPEC expression in Yersinia can be used to demonstrate H-NS-dependent regulation in this genus, we used this approach to show that H-NS modulates expression of the YmoA protein.






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