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Microbiology 154 (2008), 1318-1325; DOI  10.1099/mic.0.2007/016212-0
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Microbiology 154 (2008), 1318-1325; DOI  10.1099/mic.0.2007/016212-0
© 2008 Society for General Microbiology

Ureaplasma parvum lipoproteins, including MB antigen, activate NF-{kappa}B through TLR1, TLR2 and TLR6

Takashi Shimizu, Yutaka Kida and Koichi Kuwano

Division of Microbiology, Department of Infectious Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan

Correspondence
Koichi Kuwano
kuwano{at}med.kurume-u.ac.jp

Ureaplasma species (Ureaplasma parvum and Ureaplasma urealyticum) are commonly isolated pathogens from the female reproductive tract and are associated with perinatal diseases in humans. Inappropriate induction of inflammatory responses may be involved in the occurrence of such diseases; however, pathogenic agents that induce the inflammatory response have not been identified in ureaplasmas. In this study, we examined the involvement of Toll-like receptors (TLRs) in the activation of the immune response by U. parvum lipoproteins, as well as the U. parvum components responsible for nuclear factor {kappa}B (NF-{kappa}B) activation. The Triton X-114 (TX-114) detergent phase of U. parvum was found to induce NF-{kappa}B through TLR2. The active components of the TX-114 detergent phase were lipoproteins, such as multiple banded (MB) antigen, UU012 and UU016 of U. parvum. The activation of NF-{kappa}B by these lipoproteins was inhibited by dominant negative (DN) constructs of TLR1 and DN TLR6. Thus, the lipoproteins from U. parvum were found to activate NF-{kappa}B through TLR1, TLR2 and TLR6. Furthermore, these lipoproteins possessed an ability to induce tumour necrosis factor-alpha (TNF-{alpha}) in mouse peritoneal macrophages.

Abbreviations: DN, dominant negative; FAM20, synthetic lipopeptide containing N-terminal 20 amino acids of F0F1-type ATPase derived from Mycoplasma pneumoniae; IL, interleukin; MALP-2, macrophage-activating lipopeptide 2; MB, multiple banded; NF-{kappa}B, nuclear factor {kappa}B; Pam3CSK4, (S)-[2,3-bis(palmitoyloxy)-(2-RS)-propyl]-N-palmitoyl-(R)-Cys-(S)-Ser-(S)-Lys4-OH, 3HCl; PGN, peptidoglycan; PMF, peptide mass fingerprinting; sN-ALP2, synthesized NF-{kappa}B-activating lipopeptide 2; TLR, Toll-like receptor; TNF-{alpha}, tumour necrosis factor-alpha; TX-114, Triton X-114






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