Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 154 (2008), 1390-1399; DOI  10.1099/mic.0.2007/012641-0
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kusumoto, A.
Right arrow Articles by Homma, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kusumoto, A.
Right arrow Articles by Homma, M.
Agricola
Right arrow Articles by Kusumoto, A.
Right arrow Articles by Homma, M.
Microbiology 154 (2008), 1390-1399; DOI  10.1099/mic.0.2007/012641-0
© 2008 Society for General Microbiology

Collaboration of FlhF and FlhG to regulate polar-flagella number and localization in Vibrio alginolyticus

Akiko Kusumoto1, Akari Shinohara1,2, Hiroyuki Terashima1, Seiji Kojima1,2, Toshiharu Yakushi1,2,{dagger} and Michio Homma1,2

1 Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-Ku, Nagoya 464-8602, Japan
2 Soft Nano-Machine Project, CREST, JST, Japan

Correspondence
Michio Homma
g44416a{at}cc.nagoya-u.ac.jp

Precise regulation of the number and placement of flagella is critical for the mono-polar-flagellated bacterium Vibrio alginolyticus to swim efficiently. We have shown previously that the number of polar flagella is positively regulated by FlhF and negatively regulated by FlhG. We now show that {Delta}flhF cells are non-flagellated as are most {Delta}flhFG cells; however, some of the {Delta}flhFG cells have several flagella at lateral positions. We found that FlhF–GFP was localized at the flagellated pole, and its polar localization was seen more intensely in {Delta}flhFG cells. On the other hand, most of the FlhG–GFP was diffused throughout the cytoplasm, although some was localized at the pole. To investigate the FlhF–FlhG interaction, immunoprecipitation was performed by using an anti-FlhF antibody, and FlhG co-precipitated with FlhF. From these results we propose a model in which FlhF localization at the pole determines polar location and production of a flagellum, FlhG interacts with FlhF to prevent FlhF from localizing at the pole, and thus FlhG negatively regulates flagellar number in V. alginolyticus cells.

{dagger}Present address: Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, 8304 Minamiminowa, Nagano 399-4598, Japan.




This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
C. P. Ewing, E. Andreishcheva, and P. Guerry
Functional Characterization of Flagellin Glycosylation in Campylobacter jejuni 81-176
J. Bacteriol., November 15, 2009; 191(22): 7086 - 7093.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Balaban, S. N. Joslin, and D. R. Hendrixson
FlhF and Its GTPase Activity Are Required for Distinct Processes in Flagellar Gene Regulation and Biosynthesis in Campylobacter jejuni
J. Bacteriol., November 1, 2009; 191(21): 6602 - 6611.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
A. Kusumoto, N. Nishioka, S. Kojima, and M. Homma
Mutational Analysis of the GTP-binding Motif of FlhF which Regulates the Number and Placement of the Polar Flagellum in Vibrio alginolyticus
J. Biochem., November 1, 2009; 146(5): 643 - 650.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2008 Society for General Microbiology.