HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary data Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Cipollina, C. Articles by de Winde, J. H. PubMed PubMed Citation Articles by Cipollina, C. Articles by de Winde, J. H. Agricola Articles by Cipollina, C. Articles by de Winde, J. H.

Saccharomyces cerevisiae SFP1: at the crossroads of central metabolism and ribosome biogenesis

Chiara Cipollina^1,

¹ Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca, P.za della Scienza 2, 20126 Milano, Italy
² Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands
³ Kluyver Centre for Genomics of Industrial Fermentation, Julianalaan 67, 2628 BC Delft, The Netherlands

Correspondence
Johannes H. de Winde
j.h.dewinde{at}tudelft.nl

Saccharomyces cerevisiae SFP1 is required for nutrient-dependent regulation of ribosome biogenesis and cell size. A mutant deleted for SFP1 shows specific traits, including a slow growth phenotype, especially when growing on glucose. We recently analysed the physiology of an sfp1 mutant and its isogenic reference strain in chemostat cultures. This approach was successful in revealing the effects of nutrients on the activity of Sfp1 independent of growth rate-related feedback. In the present work we exposed carbon-limited cultures of an sfp1 mutant and its reference strain to sudden glucose excess. This allowed us to study the effect of SFP1 deletion on cell physiology when the cells are forced to exploit their maximum growth potential; this is similar to what happens in shake-flask cultures but with no bias due to growth rate differences. We show that nutrients differentiallly affect the role of Sfp1 in cell-size modulation and in transcriptional control. Furthermore, we report that while Sfp1 is necessary for the efficient glucose-dependent regulation of ribosome biogenesis genes, it is not required for the proper induction of ribosomal protein genes in response to glucose excess. Finally, our data suggest a role for Sfp1 in the regulation of glycolysis, further underlining its involvement in the network that links ribosome biogenesis and cell metabolism.

Present address: Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands.

The array data discussed in this publication have been deposited in the NCBI Gene Expression Omnibus (GEO; http://www.ncbi.nlm.nih.gov/geo/) and are accessible through GEO series accession number GSE9644.

Three supplementary tables, listing genes showing significantly different expression in the sfp1 null mutant compared with the reference strain during ethanol/glucose-limited growth, significant differences in the transcriptional response between the reference and the mutant strain following the glucose pulse, and genes in particular clusters belonging to specified functional categories, and five supplementary figures, showing the cell-size distributions, extracellular glucose concentration and specific activity of glycolytic enzymes of the two strains, the Z score profiles of the 12 clusters showing differential expression, and modulation of the budding index and cell concentration after the glucose pulse, are available with the online version of this paper.