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Microbiology 154 (2008), 1960-1972; DOI  10.1099/mic.0.2008/017525-0
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Microbiology 154 (2008), 1960-1972; DOI  10.1099/mic.0.2008/017525-0
© 2008 Society for General Microbiology

Deletion of the msdS/AfmsdC gene induces abnormal polarity and septation in Aspergillus fumigatus

Yanjie Li, Lei Zhang, Depeng Wang, Hui Zhou, Haomiao Ouyang, Jia Ming and Cheng Jin

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China

Correspondence
Cheng Jin
jinc{at}sun.im.ac.cn

{alpha}-Mannosidases play an important role in the processing of mannose-containing glycans in eukaryotes. A deficiency in {alpha}-mannosidase is lethal in humans and cattle. In contrast to mammals, Saccharomyces cerevisiae does not require the endoplasmic reticulum {alpha}-mannosidase gene for growth. However, little is known of the consequence of loss of function of class I {alpha}-mannosidases in filamentous fungi. In this study, the msdS/AfmsdC gene was identified to encode 1,2-{alpha}-mannosidase MsdS in Aspergillus fumigatus. Soluble MsdS expressed in Escherichia coli was characterized as a typical class I {alpha}-mannosidase. The msdS gene was deleted by replacement of the msdS gene with a pyrG gene. Although the mutant showed a defect in N-glycan processing, as well as a reduction of cell wall components and a reduced ability of conidiation, it appeared that the rate of hyphal growth was not affected. Morphology analysis revealed abnormal polarity and septation at the stages of germination, hyphal growth and conidiation. Although the mechanism by which the N-glycan processing affects polarity and septation is unclear, our results show that msdS is involved in polarity and septation in A. fumigatus.

Abbreviations: DAPI, 4',6-diamidino-2-phenylindole; HPAEC-PAD, high-performance anion-exchange chromatography pulsed ampere detector; LC-MS/MS, liquid chromatography/tandem MS; sMsdS, soluble MsdS

The Genbank/EMBL/DDBJ accession number for the nucleotide sequence of msdS/AfmsdC is AY573554.

A table of LC-MS/MS data is available with the online version of this paper.






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