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Microbiology 155 (2009), 61-68; DOI  10.1099/mic.0.023077-0
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Microbiology 155 (2009), 61-68; DOI  10.1099/mic.0.023077-0
© 2009 Society for General Microbiology

A phenotypic microarray analysis of a Streptococcus mutans liaS mutant

Jiaqin Zhang1,2 and Indranil Biswas1

1 Department of Microbiology, University of Kansas Medical Center, Kansas City, KS 66160, USA
2 Department of Parasitology, Shandong University School of Medicine, 44# Wenhua Xi Road, Jinan, Shandong 250012, PR China

Correspondence
Indranil Biswas
ibiswas{at}kumc.edu

Streptococcus mutans, a biofilm-forming Gram-positive bacterium that resides in the human oral cavity, is considered to be the primary aetiological agent of human dental caries. A cell-envelope stress-sensing histidine kinase, LiaS, is considered to be important for expression of virulence factors such as glucan-binding protein C and mutacin production. In this study, a liaS mutant was subjected to phenotypic microarray (PM) analysis of about 2000 phenotypes, including utilization of various carbon, nitrogen, phosphate and sulfur sources; osmolytes; metabolic inhibitors; and susceptibility to toxic compounds, including several types of antibiotics. Compared to the parental strain UA159, the liaS mutant strain (IBS148) was more tolerant to various inhibitors that target protein synthesis, DNA synthesis and cell-wall biosynthesis. Some of the key findings of the PM analysis were confirmed in independent growth studies and by using antibiotic discs and E-test strips for susceptibility testing.

Abbreviations: IM-HK, intramembrane-sensing histidine kinase; PM, phenotypic microarray; TCS, two-component signal transduction system

A supplementary table with full data from the PM analysis is available with the online version of this paper.






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