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Microbiology 155 (2009), 3294-3303; DOI  10.1099/mic.0.028795-0
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Microbiology 155 (2009), 3294-3303; DOI  10.1099/mic.0.028795-0
© 2009 Society for General Microbiology

Bifidobacterial enolase, a cell surface receptor for human plasminogen involved in the interaction with the host

Marco Candela1, Elena Biagi1, Manuela Centanni1, Silvia Turroni1, Manuela Vici2, Francesco Musiani3, Beatrice Vitali1, Simone Bergmann4, Sven Hammerschmidt5 and Patrizia Brigidi1

1 Department of Pharmaceutical Sciences, CIRB-centre for Biotechnology, University of Bologna, Italy
2 Department of Experimental Pathology, University of Bologna, Italy
3 Department of Agro Environmental Science and Technology, University of Bologna, Italy
4 Department of Microbial Pathogenicity, Helmholtz Centre for Infection Research GmbH, Braunschweig, Germany
5 Department Genetics of Microorganisms, Ernst Moritz Arndt University Greifswald, Greifswald, Germany

The interaction with the host plasminogen/plasmin system represents a novel component in the molecular cross-talk between bifidobacteria and human host. Here, we demonstrated that the plasminogen-binding bifidobacterial species B. longum, B. bifidum, B. breve and B. lactis share the key glycolytic enzyme enolase as a surface receptor for human plasminogen. Enolase was visualized on the cell surface of the model strain B. lactis BI07. The His-tagged recombinant protein showed a high affinity for human plasminogen, with an equilibrium dissociation constant in the nanomolar range. By site-directed mutagenesis we demonstrated that the interaction between the B. lactis BI07 enolase and human plasminogen involves an internal plasminogen-binding site homologous to that of pneumococcal enolase. According to our data, the positively charged residues Lys-251 and Lys-255, as well as the negatively charged Glu-252, of the B. lactis BI07 enolase are crucial for plasminogen binding. Acting as a human plasminogen receptor, the bifidobacterial surface enolase is suggested to play an important role in the interaction process with the host.

Correspondence
Patrizia Brigidi
patrizia.brigidi{at}unibo.it

Abbreviations: 2-PGE, 2-phosphoglycerate; EACA, {epsilon}-aminocaproic acid; PEP, phosphoenolpyruvate; Plg, plasminogen

The GenBank/EMBL/DDBJ accession number for the enolase sequence of B. lactis BI07 is DQ117970.






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