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Microbiology 155 (2009), 3730-3738; DOI  10.1099/mic.0.029439-0
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Microbiology 155 (2009), 3730-3738; DOI  10.1099/mic.0.029439-0
© 2009 Society for General Microbiology

Cell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckii

Pedro Antônio Castelo Teixeira1, Rafaela Alves de Castro1, Rosana Cícera Nascimento1,2, Guy Tronchin3, Armando Pérez Torres4, Márcia Lazéra5, Sandro Rogério de Almeida2, Jean-Philippe Bouchara3,6, Carla Veronica Loureiro y Penha1 and Leila M. Lopes-Bezerra1

1 Departamento de Biologia Celular, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Brazil
2 Departamento de Análises Clínicas e Toxicológicas – Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brazil
3 Groupe d'Étude des Interactions Hôte-Pathogène, UPRES-EA 3142, Université d'Angers, Angers, France
4 Laboratorio de Inmunología Comparada de Piel y Mucosas, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico
5 Instituto de Pesquisa Clínica Evandro Chagas Filho – Fiocruz, Rio de Janeiro, Brazil
6 Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Angers, France

The virulence of four Sporothrix schenckii isolates was compared in a murine model of sporotrichosis, together with the protein pattern of the yeast cell surface and the capacity to bind the extracellular matrix protein fibronectin. Virulence was determined by the mortality rate, fungal burden and histopathology. Two clinical isolates were more virulent for C57BL/6 mice, but no direct correlation was seen between virulence and the clinical or environmental origin of the isolates. The lowest virulence was observed for an isolate recovered from a patient with meningeal sporotrichosis. Although all isolates could effectively disseminate, the dissemination patterns were not similar. Using flow cytometry analysis, we investigated the interaction of all the strains with fibronectin, and showed that the binding capacity correlated with virulence. Western blot analysis of S. schenckii cell wall extracts revealed positive bands for fibronectin in the range of 37–92 kDa. The 70 kDa adhesin was also recognized by a protective monoclonal antibody raised against a gp70 antigen of S. schenckii (mAb P6E7). Confocal microscopy confirmed the co-localization of fibronectin and mAb P6E7 on the yeast cell surface. To our knowledge, this is the first report identifying adhesins for fibronectin on the surface of this human pathogen.

Correspondence
Leila M. Lopes Bezerra
leila.lopes_bezerra{at}pq.cnpq.br

Abbreviations: HRP, horseradish peroxidase






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