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Microbiology 155 (2009), 443-449; DOI  10.1099/mic.0.020826-0
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Microbiology 155 (2009), 443-449; DOI  10.1099/mic.0.020826-0
© 2009 Society for General Microbiology

The Streptococcus equi prophage-encoded protein SEQ2045 is a hyaluronan-specific hyaluronate lyase that is produced during equine infection

Anna-Marie Lindsay1, Meng Zhang1, Zoe Mitchell2, Matthew T. G. Holden3, Andrew S. Waller2, Iain C. Sutcliffe1 and Gary W. Black1

1 School of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK
2 Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, UK
3 The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK

Correspondence
Gary W. Black
gary.black{at}northumbria.ac.uk

Streptococcus equi causes equine ‘strangles’. Hyaluronate lyases, which degrade connective tissue hyaluronan and chondroitins, are thought to facilitate streptococcal invasion of the host. However, prophage-encoded hyaluronate lyases are hyaluronan-specific and are thought to be primarily involved in the degradation of the hyaluronan capsule of streptococci during bacteriophage infection. To understand the role of prophage-encoded hyaluronate lyases further, we have biochemically characterized such a hyaluronate lyase, SEQ2045 from S. equi, and have shown that it is produced during equine infection. Prophage-encoded hyaluronan-specific hyaluronate lyases may therefore play a more direct role in disease pathogenesis than previously thought.

Abbreviations: CDS, coding sequence; HA, hyaluronic acid; Hyl, hyaluronate lyase

A supplementary figure illustrating the purification of N-terminally hexahistidine-tagged SEQ2045 is available with the online version of this paper.






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