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Microbiology 155 (2009), 541-550; DOI  10.1099/mic.0.020420-0
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Microbiology 155 (2009), 541-550; DOI  10.1099/mic.0.020420-0
© 2009 Society for General Microbiology

Secreted indole serves as a signal for expression of type III secretion system translocators in enterohaemorrhagic Escherichia coli O157 : H7

Hidetada Hirakawa1,4, Toshio Kodama3, Asuka Takumi-Kobayashi1,2, Takeshi Honda3 and Akihito Yamaguchi1,2

1 Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567-0047, Japan
2 Graduate School of Pharmaceutical Science, Osaka University, Suita, Osaka 565-0871, Japan
3 Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan
4 Department of Microbiology, University of Washington, WA 98195, USA

Correspondence
Akihito Yamaguchi
akihito{at}sanken.osaka-u.ac.jp

Indole is produced by tryptophanase during growth of enteric bacteria and accumulates in the culture medium. The physiological role of indole production is poorly understood. We discovered that enterohaemorrhagic Escherichia coli (EHEC) O157 : H7 with a tnaA deletion has decreased secretion of EspA and EspB via the type III secretion system and as a result there is reduced formation of attaching and effacing (A/E) lesions in HeLa cells. Addition of indole restored and enhanced secretion of EspA and EspB and formation of A/E lesions by the tnaA deletion mutant EHEC. Indole addition moderately increased the promoter activity of LEE4 genes, including espA and espB, in the locus of enterocyte effacement. Thus in EHEC indole can serve to signal EspA and EspB expression and secretion and stimulate the ability of EHEC to form A/E lesions on human cells.

Abbreviations: A/E, attaching and effacing; EHEC, enterohaemorrhagic Escherichia coli; LEE, locus of enterocyte effacement; T3SS, type III secretion system






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