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Microbiology 155 (2009), 604-611; DOI  10.1099/mic.0.020800-0
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Microbiology 155 (2009), 604-611; DOI  10.1099/mic.0.020800-0
© 2009 Society for General Microbiology

Induction of morphological changes in Ustilago maydis cells by octyl gallate

E. Sierra-Campos1, M. A. Valdez-Solana2, D. Matuz-Mares1, I. Velázquez1 and J. P. Pardo1

1 Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Apartado Postal 70-159, Coyoacán 04510, México D. F., Mexico
2 Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, IPN, Casco de Santo Tomas, Mexico

Correspondence
J. P. Pardo
pardov{at}bq.unam.mx

The effects of octyl gallate on Ustilago maydis yeast cells were analysed in relation to its capacity to oxidize compounds (pro-oxidant actions). All phenolic compounds tested inhibited the alternative oxidase (AOX). However, only octyl gallate induced a morphological change in yeast cells and collapsed the mitochondrial membrane potential. In contrast to octyl gallate, propyl gallate and nordihydroguaiaretic acid caused only a negligible cell change and the membrane potential was not affected. Our findings show that structurally related phenolic compounds do not necessarily exert similar actions on target cells. Preincubation of U. maydis cells with trolox inhibited the change to pseudohyphal growth produced by octyl gallate. These results suggest that in addition to the inhibitory action of octyl gallate on the AOX, this compound induces a switch from yeast to a mycelium, probably through the formation of lipid peroxides.

Abbreviations: AOX, alternative oxidase; DCFDA, dichlorofluorescein diacetate; DHR123, dihydrorhodamine 123; NDGA, nordihydroguaiaretic acid; ROS, reactive oxygen species






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