Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 155 (2009), 891-902; DOI  10.1099/mic.0.022277-0
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bigot, A.
Right arrow Articles by Charbit, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bigot, A.
Right arrow Articles by Charbit, A.
Agricola
Right arrow Articles by Bigot, A.
Right arrow Articles by Charbit, A.
Microbiology 155 (2009), 891-902; DOI  10.1099/mic.0.022277-0
© 2009 Society for General Microbiology

lmo1273, a novel gene involved in Listeria monocytogenes virulence

Armelle Bigot1,2, Catherine Raynaud1,2, Iharilalao Dubail1,2, Marion Dupuis1,2, Hamid Hossain3, Torsten Hain3, Trinad Chakraborty3 and Alain Charbit1,2,{dagger}

1 Université Paris Descartes, Faculté de Médecine René Descartes, Paris F-75015, France
2 Inserm, U570, Unité de Pathogénie des Infections Systémiques, Paris F-75015, France
3 Institute for Medical Microbiology, Justus-Liebig-University, Frankfurter Strasse 107, D-35392 Giessen, Germany

Correspondence
Alain Charbit
alain.charbit{at}inserm.fr

Listeria monocytogenes is a foodborne pathogen able to infect humans and many other mammalian species, leading to serious, often fatal disease. We have previously identified a five-gene locus in the genome of L. monocytogenes EGD-e which comprised three contiguous genes encoding paralogous type I signal peptidases. In the present study, we focused on the two distal genes of the locus (lmo1272 and lmo1273), encoding proteins sharing significant similarities with the YlqF and RnhB proteins, respectively, of Bacillus subtilis. lmo1273 could complement an Escherichia coli rnhA-rnhB thermosensitive growth phenotype, suggesting that it encodes a functional RNase H. Strikingly, inactivation of lmo1273 provoked a strong attenuation of virulence in the mouse model, and kinetic studies in infected mice revealed that multiplication of the lmo1273 mutant in target organs was significantly impaired. However, the mutation did not impair L. monocytogenes intracellular multiplication or cell-to-cell spread in cell culture models. Transcriptional profiles obtained with an lmo1273-overexpressing strain were compared to those of the wild-type strain, using microarray analyses. The data obtained suggest a pleiotropic regulatory role of Lmo1273 and possible links with amino acid uptake.

Abbreviations: BMM, bone-marrow-derived macrophages; i.v., intravenous(ly); SPase I, type I signal peptidase

{dagger}Present address: Faculté de Médecine Necker, 156 rue de Vaugirard, 75730 Paris Cedex 15, France.

The microarray data associated with this paper have been deposited in the ArrayExpress database (http://www.ebi.ac.uk/arrayexpress) under the accession number E-MEXP-1162.

A supplementary table of primers and three supplementary figures are available with the online version of this paper.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2009 Society for General Microbiology.